Porous Body Preparation of Hydroxyapatite / Collagen Nanocomposites for Bone Tissue Regeneration
Porous Body Preparation of Hydroxyapatite / Collagen Nanocomposites for Bone Tissue Regeneration
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DOI：
10.4028/www.scientific.net/kem.254-256.561
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发表时间：
2003-12
期刊：
影响因子：
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通讯作者：
M. Kikuchi;T. Ikoma;D. Syoji;H. Matsumoto;Y. Koyama;S. Itoh;K. Takakuda;K. Shinomiya;M. Tanaka
中科院分区：
文献类型：
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作者：
M. Kikuchi;T. Ikoma;D. Syoji;H. Matsumoto;Y. Koyama;S. Itoh;K. Takakuda;K. Shinomiya;M. Tanaka
以胶原蛋白为粘结剂，采用冷冻干燥法制备了自组织羟基磷灰石/胶原蛋白纳米复合纤维弹性多孔体。所获得的多孔体表面快速吸附水滴，并由相互连通的孔隙组成。孔径大小取决于冷冻温度。该多孔体在润湿时表现出海绵状弹性，拉伸强度为70 kPa。通过动物实验检测的多孔体的细胞附着和骨细胞的吸收与羟基磷灰石/胶原纳米复合材料的致密体相同。这些机械和生物学性能使操作者更容易操作，并且在体外和体内比“硬”多孔陶瓷和“软”胶原海绵具有更好的细胞侵袭性。
Elastic porous bodies were fabricated from the self-organized hydroxyapatite/collagen nanocomposite fibers by lyophilization with the use of collagen as a binder. The porous bodies obtained rapidly absorbed water droplet on them and composed of interconnected pores. The pore size was depending on freezing temperatures. The porous bodies demonstrated sponge-like elastic property when wetted and 70 kPa in tensile strength. The cell attachment and resorption with osteoclastic cells for the porous bodies examined by animal tests were the same as those for the compact bodies of hydroxyapatite/collagen nanocomposite. These mechanical and biological properties provide easier handling to operator and better cell invasiveness in both in vitro and in vivo than those of "hard" porous ceramics and "soft" collagen sponges.