Development of the Honey Bee Gut Microbiome throughout the Queen-Rearing Process
Development of the Honey Bee Gut Microbiome throughout the Queen-Rearing Process
复制标题
DOI：
10.1128/aem.00307-15
复制
发表时间：
2015-02
影响因子：
4.4
通讯作者：
D. Tarpy;H. Mattila;Irene L. G. Newton
中科院分区：
文献类型：
--
作者：
D. Tarpy;H. Mattila;Irene L. G. Newton
摘要欧洲蜜蜂（Apis mellifera）被广泛用于生产蜂箱产品和作物授粉，但对蜂群健康和种群下降的普遍关注引发了对与这些重要昆虫相关的微生物群落的兴趣。目前，只有工蜂的微生物组得到了表征，而对与蚁后相关的细菌群落知之甚少，尽管它们的健康和正常功能是蜂群生产力的核心。在这里，我们提供了一个大规模的分析蜜蜂蜂王的肠道微生物组在他们的发展轨迹，并通过多个殖民地，主机他们作为现代女王饲养实践的一部分。我们发现，随着年龄的增长以及遇到不同的工蜂种群和菌落环境，蜂王微生物组的大小和组成发生了巨大的变化。女王微生物组在早期生活中主要由肠道细菌组成，但在成熟时主要由α-变形菌组成。此外，女王肠道微生物组并没有反映出照顾它们的工人的微生物组，事实上，它们缺乏许多被认为对工人至关重要的细菌。虽然工人的肠道微生物是一致的不相关的殖民地人口采样，微生物的相关皇后是高度可变的。成熟的皇后肠道中的细菌群落的大小相似的成熟的工人，其特征在于显性和特定的alphaproteobacterial菌株已知与工人下咽腺。我们的研究结果提出了一个模型，其中皇后肠道被来自工人腺体的细菌定殖，与其他动物微生物组的母体接种途径相反。
ABSTRACT The European honey bee (Apis mellifera) is used extensively to produce hive products and for crop pollination, but pervasive concerns about colony health and population decline have sparked an interest in the microbial communities that are associated with these important insects. Currently, only the microbiome of workers has been characterized, while little to nothing is known about the bacterial communities that are associated with queens, even though their health and proper function are central to colony productivity. Here, we provide a large-scale analysis of the gut microbiome of honey bee queens during their developmental trajectory and through the multiple colonies that host them as part of modern queen-rearing practices. We found that queen microbiomes underwent a dramatic shift in size and composition as they aged and encountered different worker populations and colony environments. Queen microbiomes were dominated by enteric bacteria in early life but were comprised primarily of alphaproteobacteria at maturity. Furthermore, queen gut microbiomes did not reflect those of the workers who tended them and, indeed, they lacked many of the bacteria that are considered vital to workers. While worker gut microbiotas were consistent across the unrelated colony populations sampled, the microbiotas of the related queens were highly variable. Bacterial communities in mature queen guts were similar in size to those of mature workers and were characterized by dominant and specific alphaproteobacterial strains known to be associated with worker hypopharyngeal glands. Our results suggest a model in which queen guts are colonized by bacteria from workers' glands, in contrast to routes of maternal inoculation for other animal microbiomes.