OsMYB45 plays an important role in rice resistance to cadmium stress
OsMYB45 plays an important role in rice resistance to cadmium stress
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OsMYB45在水稻抗镉胁迫中发挥重要作用
DOI：
10.1016/j.plantsci.2017.08.002
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发表时间：
2017-11-01
期刊：
影响因子：
5.2
通讯作者：
Zheng, Luqing
中科院分区：
文献类型：
--
作者：
Hu, Shubao;Yu, Yao;Zheng, Luqing
镉(Cd)是自然界中毒性最强的重金属元素之一，对植物细胞造成严重危害。在这里，我们报道了一个转录因子OsMYB45参与了水稻对Cd胁迫的反应。OsMYB45在水稻叶片、壳、雄蕊、雌蕊和侧根中高表达，其表达受Cd胁迫诱导。在洋葱表皮细胞中，OsMYB45与绿色荧光蛋白融合，定位于细胞核。OsMYB45突变株对Cd处理敏感，叶片中H_2O_2浓度较野生型增加近一倍，过氧化氢酶(CAT)活性降低一半。基因表达分析表明，OsCATA和OsCATC在突变体中的表达显著低于野生型。此外，OsMYB45在突变体中的过表达补充了突变体的表型。综上所述，OsMYB45在水稻对Cd胁迫的耐性中起着重要作用。
Cadmium (Cd) is one of the most toxic heavy metal elements in nature, and it causes serious damage to plant cells. Here, we report that a transcription factor OsMYB45 is involved in Cd stress response in rice. OsMYB45 is highly expressed in rice leaves, husks, stamens, pistils, and lateral roots, and its expression is induced by Cd stress. OsMYB45 fused to green fluorescent protein localized to the cell nucleus in onion epidermal cells. Mutation of OsMYB45 resulted in hypersensitivity to Cd treatment, and the concentration of H2O2 in the leaves of mutant nearly doubled, while catalase (CAT) activity was halved compared with the wild-type. Moreover, gene expression analysis indicated that OsCATA and OsCATC expression is significantly lower in the mutant than in the wild-type. In addition, overexpression of OsMYB45 in the mutant complemented the mutant phenotype. Taken together, OsMYB45 plays an important role in tolerance to Cd stress in rice.