ClRTL1 Encodes a Chinese Fir RNase III-Like Protein Involved in Regulating Shoot Branching.
ClRTL1 Encodes a Chinese Fir RNase III-Like Protein Involved in Regulating Shoot Branching.
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DOI：
10.3390/ijms161025691
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发表时间：
2015-10-26
影响因子：
5.6
通讯作者：
Shi J
中科院分区：
文献类型：
--
作者：
Li X;Su Q;Zheng R;Liu G;Lu Y;Bian L;Chen J;Shi J
鉴定控制分枝的基因对于改善植物结构和提高作物产量或生物量至关重要。杉木分枝突变体“都甘沙”（Cunninghamia lanceolata var. dugan.）在我们的实验室里被分离出来我们选择了cDNA-AFLP技术和一个有效的策略来筛选潜在的调控杉木枝条分枝的基因，利用该突变体。命名为ClRTL 1的RNase III样1 cDNA片段被鉴定为潜在的正调节因子。为了研究ClRTL 1基因在植物分枝调控中的功能，我们从C. lanceolata（Lamb.）Hook，推导出其二级结构和功能，并在拟南芥中过量表达。ClRTL 1 cDNA全长1045 bp，包含一个705 bp的开放阅读框。它编码235个氨基酸的蛋白质。ClRTL 1的二级结构表明它是一种类似于mini-RNase III的蛋白。35 S：ClRTL 1基因在A. thaliana的结果表明，ClRTL 1在促进杉木枝梢分枝中起着重要作用。
Identification of genes controlling shoot branching is crucial for improving plant architecture and increasing crop yield or biomass. A branching mutant of Chinese fir named “Dugansha” (Cunninghamia lanceolata var. dugan.) has been isolated in our laboratory. We chose the cDNA-AFLP technique and an effective strategy to screen genes that potentially regulate shoot branching in Chinese fir using this mutant. An RNase III-like1 cDNA fragment named ClRTL1 was identified as a potential positive regulator. To investigate the function of ClRTL1 in regulating shoot branching, we cloned the full-length cDNA sequence from C. lanceolata (Lamb.) Hook, deduced its secondary structure and function, and overexpressed the coding sequence in Arabidopsis. The ClRTL1 cDNA is 1045 bp and comprises an open reading frame of 705 bp. It encodes a protein of 235 amino acids. The deduced secondary structure of the ClRTL1 indicates that it is a mini-RNase III-like protein. The expression analysis and phenotypes of 35S: ClRTL1 in A. thaliana implies that ClRTL1 plays a role in promoting shoot branching in Chinese fir.