Effects of simulated gastroesophageal reflux on the untraumatized rabbit larynx.
Effects of simulated gastroesophageal reflux on the untraumatized rabbit larynx.
复制标题
模拟胃食管反流对未受损伤的兔喉的影响。
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
A. Al
中科院分区：
文献类型：
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作者：
J. Ludemann;J. Manoukian;K. Shaw;C. Bernard;M. Davis;A. Al
目标
临床和实验表明，胃食管反流（GER）会导致受伤的喉粘膜发炎。本研究旨在确定 GER 是否会导致未受损伤的喉粘膜发炎。
方法
16只成年新西兰白兔在全身麻醉下进行管状咽造口术，无需气管插管。恢复7天后，在轻度镇静状态下将1 mL/kg生理盐水或HCl（pH 1.5）加胃蛋白酶（0.3 mg/mL）注入梨状窦：每天1次、2次或3次，连续14天。
结果
接受盐酸和胃蛋白酶的兔子表现出不同程度的呼吸暂停和咳嗽。最终，大多数人在用力时出现双相喘鸣。从组织学角度来看，每天模拟3次GER时喉部炎症程度最严重。一只兔子的声门炎症最严重，它对 GER 模拟的反应是阵发性咳嗽。
结论
GER 喉炎的损伤机制被认为是化学损伤（由于 HCl 和胃蛋白酶）和机械损伤（由于个体可变的喉部化学反射）的组合。有必要在这种新的动物模型中进一步研究 GER 喉炎。
OBJECTIVE
Gastroesophageal reflux (GER) has been shown, clinically and experimentally, to cause inflammation of traumatized laryngeal mucosa. This study was performed to determine if GER causes inflammation of untraumatized laryngeal mucosa.
METHOD
Sixteen adult New Zealand white rabbits underwent tube pharyngostomy under general anaesthesia without endotracheal intubation. After 7 days recovery, 1 mL/kg of normal saline or HCl (pH 1.5) with pepsin (0.3 mg/mL) was infused into the piriform sinus while the rabbit was under mild sedation: once, twice, or three times per day, for 14 consecutive days.
RESULTS
Rabbits that received HCl and pepsin exhibited various degrees of apnea and coughing. Eventually, most developed biphasic stridor on exertion. Histologically, the degree of laryngeal inflammation was greatest when GER was simulated three times per day. Glottic inflammation was greatest in one rabbit, which responded to GER simulation with paroxysmal coughing.
CONCLUSION
The mechanism of injury in GER laryngitis is postulated to be a combination of chemical trauma (due to HCl and pepsin) and mechanical trauma (due to an individually variable laryngeal chemoreflex). Further study of GER laryngitis in this new animal model is warranted.