Optofluidic wavelength division multiplexing for single-virus detection
Optofluidic wavelength division multiplexing for single-virus detection
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DOI：
10.1073/pnas.1511921112
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发表时间：
2015-10-20
影响因子：
11.1
通讯作者：
Schmidt, Holger
中科院分区：
文献类型：
--
作者：
Ozcelik, Damla;Parks, Joshua W.;Schmidt, Holger
光波导同时传输不同颜色的光，形成了光纤电信网络的基础，该网络在数十个光谱分离的通道中穿梭数据。在这里，我们重新想象这种波分复用（WDM）的范例在一个新的背景下，有区别的检测和识别的单一流感病毒的芯片。我们使用一个单一的多模干涉（MMI）波导在整个可见光谱范围内创建波长相关的光斑图案，并在光流控芯片上实现多路复用的单个生物分子检测。每个目标通过其时间依赖性荧光信号来识别，而不需要在检测时进行光谱解复用。我们证明了在两种实现方式中检测三种甲型流感亚型的单个荧光标记病毒颗粒：使用三种不同颜色标记每种病毒和双色组合标记。通过将组合多路复用扩展到三种或更多种颜色，基于MMI的WDM提供了差异化临床测试和日益增长的个性化医疗领域所需的多路复用能力。
Optical waveguides simultaneously transport light at different colors, forming the basis of fiber-optic telecommunication networks that shuttle data in dozens of spectrally separated channels. Here, we re-imagine this wavelength division multiplexing (WDM) paradigm in a novel context-the differentiated detection and identification of single influenza viruses on a chip. We use a single multimode interference (MMI) waveguide to create wavelength-dependent spot patterns across the entire visible spectrum and enable multiplexed single bio-molecule detection on an optofluidic chip. Each target is identified by its time-dependent fluorescence signal without the need for spectral demultiplexing upon detection. We demonstrate detection of individual fluorescently labeled virus particles of three influenza A subtypes in two implementations: labeling of each virus using three different colors and two-color combinatorial labeling. By extending combinatorial multiplexing to three or more colors, MMI-based WDM provides the multiplexing power required for differentiated clinical tests and the growing field of personalized medicine.