Generation of chitosan nanoporous structures for tissue engineering applications using a supercritical fluid assisted process
Generation of chitosan nanoporous structures for tissue engineering applications using a supercritical fluid assisted process
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DOI：
10.1016/j.supflu.2010.05.014
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发表时间：
2010-09-01
影响因子：
3.9
通讯作者：
Reverchon, E.
中科院分区：
文献类型：
--
作者：
Cardea, S.;Pisanti, P.;Reverchon, E.
近年来，壳聚糖基材料及其在组织工程领域的应用受到了极大的关注。然而，目前提出的壳聚糖支架的制备技术存在一些局限性，如耗时长、使用有机溶剂、难以获得和保存不同层次的孔隙度和三维结构等。本文提出了一种新的超临界CO2辅助制备壳聚糖支架的工艺，该工艺包括三个步骤：通过热诱导相分离形成壳聚糖水凝胶；用合适的溶剂取代水；用超临界CO2干燥凝胶。利用这种工艺，我们得到了纤维直径约50 nm的壳聚糖纳米结构，凝胶纳米结构没有任何坍塌，具有高孔隙率(>；91%)和高压缩模数(150kpa)的特点。(C)2010爱思唯尔B.V.保留所有权利。
In recent years, considerable attention has been given to chitosan-based materials and their applications in the field of tissue engineering. However, the techniques proposed until now for the formation of chitosan scaffolds present some limitations such as: they are very time-consuming, use organic solvents, have difficulties in the obtainment and preservation of various levels of porosity and the 3-D structure. In this work, a new SC-CO2 assisted process for the production of chitosan scaffolds is proposed; it consists of three steps: formation of a chitosan hydrogel by thermally induced phase separation; substitution of water with a suitable solvent; drying of the gel using SC-CO2. Using this process, we produced chitosan nanostructured networks with filaments diameters around 50 nm, without any collapse of the gel nanostructure, characterized by a high porosity (>91%) and high compressive modulus (150 kPa). (C) 2010 Elsevier B.V. All rights reserved.