The Caenorhabditis elegans and Haemonchus contortus beta-tubulin genes cannot substitute for loss of the Saccharomyces cerevisiae beta-tubulin gene.
The Caenorhabditis elegans and Haemonchus contortus beta-tubulin genes cannot substitute for loss of the Saccharomyces cerevisiae beta-tubulin gene.
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DOI：
10.17912/micropub.biology.000411
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发表时间：
2021-06-30
影响因子：
--
通讯作者：
Andersen EC
中科院分区：
文献类型：
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作者：
Gibson SB;Harper CS;Lackner LL;Andersen EC
为了更好地理解由β-微管蛋白和苯并咪唑化合物之间的假定相互作用引起的抗性机制，我们试图在替换单个酿酒酵母β-微管蛋白基因后使用异源表达来纯化线虫特异性β-微管蛋白。然而，我们发现含有线虫特异性β-微管蛋白基因的单倍体酵母细胞不能存活，这表明线虫β-微管蛋白不能替代酵母β-微管蛋白基因的缺失。
To better understand the mechanism of resistance caused by putative interactions between beta-tubulin and benzimidazole compounds, we sought to purify nematode-specific beta-tubulins using heterologous expression after replacement of the single Saccharomyces cerevisiae beta-tubulin gene. However, we found that haploid yeast cells containing nematode-specific beta-tubulin genes were not viable, suggesting that nematode beta-tubulin cannot substitute for the loss of the yeast beta-tubulin gene.