[The Future Topics of Discussion on JSCC Recommended Methods].
[The Future Topics of Discussion on JSCC Recommended Methods].
复制标题
[JSCC推荐方法的未来讨论主题]。
DOI：
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
T. Nakayama
中科院分区：
文献类型：
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作者：
S. Yamadate;T. Nakayama
JSCC自1989年制定AST、ALT、CK、LD活性测定推荐方法以来，已提出了8项酶和9项非酶（Na、K、Cl一项）的推荐方法。这些推荐方法是根据当时日本测定方法的实际情况制定的，与IFCC推荐方法或主要参考程序有一定差异。近年来，从全球标准化的角度来看，即使在临床检查中也需要能够与海外数据共享的测量值，有时会使用海外检查设施来避免国际临床试验中测量方法的差异。鉴于这种情况，当比较JSCC和IFCC推荐的方法时，存在以下差异： - AST和ALT的PALP：JSCC方法中未添加/IFCC方法中添加。 - LD 缓冲溶液 (pH)：JSCC 方法中的 EAE (pH 8.8)/IFCC 方法中的 NMG (pH 9.4)。 ALP 缓冲液：JSCC 方法中的 EAE/IFCC 方法中的 AMP。 TG 游离甘油：不包含在 JSCC 方法中/包含在 NIST 和 CDC 的 ID-GC/MS 方法中。这些差异是否影响全球标准化的实施，需要从多方面考虑。考虑到，有必要在国内监测中增加该方法应如何直接与CRMLN（胆固醇参考方法实验室网络）和JCTLM（检验医学溯源联合委员会）国际溯源挂钩的观点并进行判断。 [审查]。
In the JSCC, recommended methods for eight items of enzymes and 9 items of non-enzyme (one item for Na, K, and Cl) have been presented since the recommended methods of activity measurements of AST, ALT, CK, and LD were prepared in 1989. These recommended methods were prepared by placing priority on the actual situation of the measurement methods of those days in Japan, and there are some differences with IFCC recommended methods or primary reference procedures. Recently, from the viewpoint of global standardization, measured values that can be shared with data overseas are required even on clinical exami- nation, and overseas inspection facilities are sometimes used to avoid differences in measurement methods in international clinical trials. With this situation, when the recommended methods of JSCC and IFCC are compared, there are the following differences: - PALP for AST and ALT: not added in JSCC method/ added in IFCC method. - Buffer solution (pH) for LD: EAE (pH 8.8) in JSCC method/ NMG (pH 9.4) in IFCC method. Buffer solution for ALP: EAE in JSCC method/ AMP in IFCC method. Free glycerol for TG: not included on JSCC method/ included in ID-GC/MS method of NIST and CDC. It is necessary to consider from various aspects whether or not these differences affect the implementation of global standardization. In consideration, it is necessary to add the point of how the method should be linked directly to the international traceability of CRMLN (Cholesterol Reference Method Laboratory Net- work) and JCTLM (Joint Committee for Traceability in Laboratory Medicine) to the domestic surveillance and to judge it. [Review].