The psbA gene of DCMU-resistant Euglena gracilis has an amino acid substitution at serine codon 265.
The psbA gene of DCMU-resistant Euglena gracilis has an amino acid substitution at serine codon 265.
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DCMU 抗性眼虫的 psbA 基因在丝氨酸密码子 265 处有氨基酸取代。
DOI：
10.1007/bf00434825
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发表时间：
1987
期刊：
影响因子：
2.5
通讯作者：
Hallick,RB
中科院分区：
文献类型：
--
作者：
Johanningmeier,U;Hallick,RB
编码光系统II的除草剂结合QB蛋白的psbA基因先前已被测序（Karabin等，1984）。对一株抗除草剂的裸藻突变体Euglena gracilisZR的psbA基因及其mRNA进行了部分序列测定。测序反应通过使psbA特异性末端标记的DNA-寡聚体与总叶绿体DNA或RNA退火并在四种双脱氧核苷酸存在下用逆转录酶延伸该引物来进行。在位置265处检测到从丝氨酸到丙氨酸的氨基酸取代。迄今为止测序的所有已知的抗除草剂高等植物和衣原体突变体DCMU-4在完全相同的位置显示出变化，但高等植物中的取代是从丝氨酸到甘氨酸。
The psbA gene coding for the herbicide binding QBprotein of photosystem II has been sequenced previously (Karabin et al. 1984). A herbicide resistant mutant of Euglena,Euglena gracilisZR, was studied by sequencing part of the psbA gene and its corresponding mRNA. Sequencing reactions were done by annealing a psbA specific, end-labeled DNA-ohgomer to total chloroplast DNA or RNA and extending this primer with reverse transcriptase in the presence of the four dideoxynucleotides. An amino acid substitution from serine to alanine at position 265 was detected. All known herbicide resistant higher plants sequenced to date and theChlamydomonasmutant DCMU-4 show a change at exactly this same position, but the substitution in higher plants is from serine to glycine.