Chemolithoautotrophic oxidation of thiosulfate and phylogenetic distribution of sulfur oxidation gene (soxB) in rhizobacteria isolated from crop plants
Chemolithoautotrophic oxidation of thiosulfate and phylogenetic distribution of sulfur oxidation gene (soxB) in rhizobacteria isolated from crop plants
复制标题
DOI：
10.1016/j.resmic.2008.08.007
复制
发表时间：
2008-11-01
影响因子：
2.6
通讯作者：
Sa, Tong Min
中科院分区：
文献类型：
--
作者：
Anandham, Rangasamy;Indiragandhi, Pandiyan;Sa, Tong Min
从根际土壤中分离出21株硫代硫酸盐氧化细菌，16S rRNA分析表明，这些菌株隶属于变形菌门和放线菌门的Beta和Gamma亚类中的7个不同的系统发育群。其中Dyella、Burkholderia、Alcaligenes、Microbacterium和Leifsonia sp.等5个属，代表了根际土壤中新的硫氧化剂。硫代硫酸盐氧化的Dyella，伯克霍尔德氏菌，产碱菌，微杆菌，Leifsonia和Pandoraea能够生长chemolithotrophically与含硫代硫酸盐的培养基，并表现出与硫代硫酸盐氧化的增长。在硫代硫酸盐氧化的过程中，它们在废培养基中积累中间产物，如硫、亚硫酸盐和连三硫酸盐，并且这些产物最终被氧化成硫酸盐。此外，它们还具有硫代硫酸盐代谢酶，如罗丹酸酶、硫代硫酸盐氧化酶、亚硫酸盐氧化酶和连三硫酸盐水解酶，这表明这些细菌使用“S4中间体”（S4I）途径进行硫代硫酸盐氧化。soxB基因的系统发育分析表明，Pandoraea sp.和Pandoraea pnomenusa菌株在Betaproteobacteria中形成了一个独立的谱系。(C)2008年，Elsevier Masson SAS。All rights reserved.
Twenty-one thiosulfate-oxidizing bacteria were isolated from rhizosphere soils and 16S rRNA analysis revealed that the isolates were affiliated with seven different phylogenetic groups within the Beta and Gamma subclasses of Proteobacteria and Actinobacteria. Among these, five genera, including Dyella, Burkholderia, Alcaligenes, Microbacterium and Leifsonia sp., represented new sulfur oxidizers in rhizosphere soils. The thiosulfate-oxidizing Dyella, Burkholderia, Alcaligenes, Microbacterium, Leifsonia and Pandoraea were able to grow chemolithotrophically with a medium containing thiosulfate and exhibited growth coupled with thiosulfate oxidation. They accumulated intermediate products such as sulfur, sulfite and trithionate in the spent medium during the time course of thiosulfate oxidation, and these products were finally oxidized into sulfate. Furthermore, they possessed thiosulfate-metabolizing enzymes such as rhodanese, thiosulfate oxidase, sulfite oxidase and trithionate hydrolase, suggesting that these bacteria use the 'S4 intermediate' (S4I) pathway for thiosulfate oxidation. Phylogenetic analysis of the soxB gene revealed that Pandoraea sp. and Pandoraea pnomenusa strains formed a separate lineage within Betaproteobacteria. (C) 2008 Elsevier Masson SAS. All rights reserved.