Methods for gene cloning and targeted mutagenesis.
Methods for gene cloning and targeted mutagenesis.
复制标题
基因克隆和靶向突变的方法。
DOI：
10.1007/978-1-60327-365-7_12
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Rood,JulianI
中科院分区：
文献类型：
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作者：
Carter,GlenP;Lyras,Dena;Poon,Rachael;Howarth,PaulineM;Rood,JulianI
艰难梭菌是一系列肠道疾病的病原体，统称为艰难梭菌相关疾病（CDAD）。最近出现的与人类死亡率和疾病严重程度增加相关的“高毒力”菌株突出了在分子水平上研究这种生物体的必要性。这些研究将增加我们对C.困难的疾病，并促进新的和改进的治疗的合理设计。对C. difficile长期以来一直受到遗传操纵有机体的困难的阻碍。直到最近（在过去的十年中）才开发出将质粒DNA导入C.困难，最重要的是，使同基因突变体的产生，在这种新兴的人类病原体。这些方法是有效研究这种重要细菌中基因功能的必要前提。
Clostridium difficileis the causative agent of a range of intestinal diseases, collectively referred to asClostridium difficile-associated disease (CDAD). The recent emergence of “hypervirulent” strains associated with increased rates of mortality and severity of disease in humans has highlighted the need to study this organism at the molecular level. These studies will increase our knowledge of the mechanisms by whichC. difficilecauses disease and facilitate the rational design of new and improved therapeutics. The study ofC. difficilehas long been hampered by difficulties in genetically manipulating the organism. It has been only recently (within the last decade) that methods have been developed to introduce plasmid DNA intoC. difficileand most importantly to enable the generation of isogenic mutants in this emerging human pathogen. These methods are essential prerequisites for the effective study of gene function in this important bacterium.