Requirements of Lgl in cell differentiation and motility during Drosophila ovarian follicular epithelium morphogenesis
Requirements of Lgl in cell differentiation and motility during Drosophila ovarian follicular epithelium morphogenesis
复制标题
果蝇卵巢滤泡上皮形态发生过程中Lgl对细胞分化和运动的要求
DOI：
10.4161/fly.5.2.14436
复制
发表时间：
2011-04-01
期刊：
影响因子：
1.2
通讯作者：
Li, Mingfa
中科院分区：
文献类型：
--
作者：
Li, Qi;Feng, Shiyun;Li, Mingfa
果蝇卵室上皮滤泡细胞层在卵子发生时经历了形态发生和图案形成过程。这些发育过程对于构建蛋壳和建立蛋和所得胚胎的体轴是必不可少的，因此对于蛋的发育至关重要。我们先前已经证明，致死（2）巨大幼虫（lgl），果蝇肿瘤抑制基因（nTSG）是滤泡上皮前后模式形成过程中后滤泡细胞（PFC）命运诱导所必需的。在这份报告中，我们进一步描述了lgl在这种上皮模式和特定边缘细胞的形态发生变化。lgl与大圆盘（dlg）和scribble（scrib）的遗传相互作用，另外两个nTSGs在指定PFC命运中揭示了该组基因的合作作用。同时，我们发现lgl功能的丧失导致了卵泡前部细胞的分化失败。克隆分析进一步表明，lgl不仅是必要的边缘细胞的分化，而且可能是通过调节顶-基底极性和细胞粘附控制集体边缘细胞迁移。总的来说，我们确定Lgl作为一个重要的因素，在调节分化和形态发生运动的卵巢上皮卵泡细胞。
The epithelial follicle cell layer over the egg chamber in Drosophila ovary undergoes patterning and morphogenesis at oogenesis. These developmental processes are essential for constructing the eggshell and establishing the body axes of the egg and resultant embryo, thereby being crucial for the egg development. We have previously shown that lethal(2)giant larvae (lgl), a Drosophila neoplastic tumor suppressor gene (nTSG) is required for the posterior follicle cell (PFC) fate induction during antero-posterior pattern formation of the follicular epithelium. In this report, we further characterize lgl in this epithelium patterning and the morphogenetic changes of specified border cells. Genetic interactions of lgl with discs large (dlg) and scribble (scrib), another two nTSGs in specifying the PFC fate reveal a cooperative role of this group of genes. Meanwhile, we find that loss of lgl function causes failure of follicle cells at the anterior to differentiate properly. The clonal analysis further indicates that lgl is necessary not only for the border cell differentiation, but also for control of the collective border cell migration via presumably modulating the apico-basal polarity and cell adhesion. Overall, we identify Lgl as an essential factor in regulating differentiation and morphogenetic movement of the ovarian epithelial follicle cells.