Isolation and Culture Properties of a Thermophilic Agarase-Producing Strain, Microbulbifer sp. SD-1
Isolation and Culture Properties of a Thermophilic Agarase-Producing Strain, Microbulbifer sp. SD-1
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嗜热琼脂酶产生菌株 Microbulbifer sp. 的分离和培养特性。
DOI：
10.5657/fas.2011.0186
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发表时间：
2011
影响因子：
--
通讯作者：
Eun
中科院分区：
文献类型：
--
作者：
Do;Yumi Jang;Kyoung;Mi Na Lee;A. Kim;Eun;Tae;E. Jeong;H. Kwon;Byung;Eun
从海水中分离到一株琼胶降解酶产生菌。经16 S rRNA序列分析，该菌株被鉴定为Microbulbifer sp. SD-1。最适生长pH为6.0，最适生长温度为30°C，最适生长pH为9.0，最适生长温度为60°C。该分离物需要5%NaCl的最佳生长，并显示45%的生长活性，没有NaCl。培养基中0-0.4%的琼脂浓度不影响生长。薄层层析分析表明，该菌株能将琼脂降解为单糖和寡糖，具有工业应用价值。
An agar-degrading enzyme-producing strain was isolated from seawater. The isolate was identified as Microbulbifer sp. SD-1 by 16S rRNA sequencing analysis. The optimal pH and temperature for growth were 6.0 and 30°C, respectively, and growth was pos- sible at pH 9.0 and 60°C. The isolate required 5% NaCl for optimal growth and showed 45% growth activity without NaCl. Agar concentrations of 0-0.4% in the medium did not affect growth. Thin-layer chromatography analysis revealed that this strain could degrade agar into a monosaccharide and oligosaccharide, which may have industrial applications.