Pattern formation in Escherichia coli: A model for the pole-to-pole oscillations of Min proteins and the localization of the division site
Pattern formation in Escherichia coli: A model for the pole-to-pole oscillations of Min proteins and the localization of the division site
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DOI：
10.1073/pnas.251216598
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发表时间：
2001-12-04
影响因子：
11.1
通讯作者：
de Boer, PAJ
中科院分区：
文献类型：
--
作者：
Meinhardt, H;de Boer, PAJ
正确的细胞分裂需要准确定义分裂平面。在细菌中，该平面由FtsZ蛋白的聚合环决定。Z环组装的网站反过来是由最小的系统，它抑制FtsZ聚合在非中心膜网站控制。大肠杆菌中的Min蛋白经历高度动态的定位周期，在此期间它们在两个细胞半体的膜之间振荡。通过使用计算机模拟，我们表明，通过使用以下假设，可以准确地描述Min蛋白质动力学：（i）MinD ATP酶在膜上自组装并募集MinC（Z环形成的抑制剂）和MinE（MinC/MinD振荡所需的蛋白质），（ii）MinE的局部积累是通过基于局部自增强和长程拮抗效应的模式形成反应产生的，并且（拟合）它从膜中置换MinD，导致其自身的局部不稳定并向更高的MinD浓度转移。这种局部不稳定导致高MinE浓度的波从电池中心行进到极点，在那里它消失。MinD在另一半细胞的膜上重新组装，并吸引新的MinE积累，导致MinD再次波浪式分解。结果是MinC/D的极间振荡。在时间平均上，MinC浓度在两极最高，迫使FtsZ组装到中心。该机制是自组织的，不需要任何其他假设的拓扑决定因素。
Proper cell division requires an accurate definition of the division plane. In bacteria, this plane is determined by a polymeric ring of the FtsZ protein. The site of Z ring assembly in turn is controlled by the Min system, which suppresses FtsZ polymerization at noncentral membrane sites. The Min proteins in Escherichia coli undergo a highly dynamic localization cycle, during which they oscillate between the membrane of both cell halves. By using computer simulations we show that Min protein dynamics can be described accurately by using the following assumptions: (i) the MinD ATPase self-assembles on the membrane and recruits both MinC, an inhibitor of Z ring formation, and MinE, a protein required for MinC/MinD oscillation, (it) a local accumulation of MinE is generated by a pattern formation reaction that is based on local self-enhancement and a long range antagonistic effect, and (fit) it displaces MinD from the membrane causing its own local destabilization and shift toward higher MinD concentrations. This local destabilization results in a wave of high MinE concentration traveling from the cell center to a pole, where it disappears. MinD reassembles on the membrane of the other cell half and attracts a new accumulation of MinE, causing a wave-like disassembly of MinD again. The result is a pole-to-pole oscillation of MinC/D. On time average, MinC concentration is highest at the poles, forcing FtsZ assembly to the center. The mechanism is self-organizing and does not require any other hypothetical topological determinant.