Site-matched assessment of structural and tissue properties of cortical bone using scanning acoustic microscopy and synchrotron radiation μCT
Site-matched assessment of structural and tissue properties of cortical bone using scanning acoustic microscopy and synchrotron radiation μCT
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DOI：
10.1088/0031-9155/51/3/017
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发表时间：
2006-02-07
影响因子：
3.5
通讯作者：
Laugier, P
中科院分区：
文献类型：
--
作者：
Raum, K;Leguerney, I;Laugier, P
使用200 MHz扫描声学显微镜（SAM）和同步辐射mu CT（SR-mu CT）评估皮质骨中感兴趣的部位匹配区域的显微结构参数和组织特性。对10个人桡骨皮质横截面的前、后外侧区域进行了探索。使用定制开发的图像融合和分析软件，用两种方法评估结构参数，包括每个皮质区域的哈弗氏管直径和数量（Ca.Dm，N.Ca/Ar）和孔隙度Po。从融合图像中分别提取骨细胞和间质组织的声阻抗Z和骨DMB矿化度。从射线照相和声学图像获得的结构参数估计几乎相同。DMB和阻抗值分别在0.77和1.28 g cm（-3）以及5.13和12.1 Mrayl之间。观察到个体间和区域间的差异，而骨细胞和间质组织之间的差异最大（Z：7.2 +/- 1.1 Mrayl vs 9.3 +/- 1.0 Mrayl，DMB：1.06 +/- 0.07 g cm（-3）vs 1.16 0.05 g cm（-3），配对t检验，p < 0.05）。对于骨细胞（R-2 = 0.174，p < 10（-4））和合并（骨细胞和间质）数据，DMB和Z之间存在微弱但显著的相关性。合并的骨细胞和间质组织数据的回归遵循二阶多项式（R-2 = 0.39，p < 10（-4））。两种模式都满足在组织水平上同时评价皮质骨微观结构和材料特性的要求。虽然SAM检查仅限于对精心准备的样品表面进行评估，但SR-mu CT可提供组织的体积信息，而无需大量准备要求。然而，SAM提供了在组织水平上的弹性特性的定量估计，这是SR-mu CT无法捕获的。
200 MHz scanning acoustic microscopy (SAM) and synchrotron radiation mu CT (SR-mu CT) were used to assess microstructural parameters and tissue properties in site-matched regions of interest in cortical bone. Anterior and postero-lateral regions of ten cross sections from human cortical radius were explored. Structural parameters, including diameter and number of Haversian canals per cortical area (Ca.Dm, N.Ca/Ar) and porosity Po were assessed with both methods using a custom-developed image fusion and analysis software. Acoustic impedance Z and degree of mineralization of bone DMB were extracted separately for osteonal and interstitial tissues from the fused images. Structural parameter estimations obtained from radiographic and acoustic images were almost identical. DMB and impedance values were in the range between 0.77 and 1.28 g cm(-3) and 5.13 and 12.1 Mrayl, respectively. Interindividual and regional variations were observed, whereas the strongest difference was found between osteonal and interstitial tissues (Z: 7.2 +/- 1.1 Mrayl versus 9.3 +/- 1.0 Mrayl, DMB: 1.06 +/- 0.07 g cm(-3) versus 1.16 0.05 g cm(-3), paired t-test, p < 0.05). Weak, but significant correlations between DMB and Z were obtained for the osteonal (R-2 = 0.174, p < 10(-4)) and for the pooled (osteonal and interstitial) data. The regression of the pooled osteonal and interstitial tissue data follows a second-order polynomial (R-2 = 0.39, p < 10(-4)). Both modalities fulfil the requirement for a simultaneous evaluation of cortical bone microstructure and material properties at the tissue level. While SAM inspection is limited to the evaluation of carefully prepared sample surfaces, SR-mu CT provides volumetric information on the tissue without substantial preparation requirements. However, SAM provides a quantitative estimate of elastic properties at the tissue level that cannot be captured by SR-mu CT.