Human embryonal carcinoma stem cells: Models of embryonic development in humans
Human embryonal carcinoma stem cells: Models of embryonic development in humans
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DOI：
10.1089/1547328041797507
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发表时间：
2004-08-01
影响因子：
4
通讯作者：
Horrocks, GM
中科院分区：
文献类型：
--
作者：
Przyborski, SA;Christie, VB;Horrocks, GM
有几个可靠的实验系统可用于研究控制人类胚胎发育的分子机制。胚胎癌（EC）细胞是来源于畸胎瘤的多能干细胞，并且被认为是人类胚胎干（ES）细胞的恶性对应物。几种现有的人EC干细胞系提供了稳健和简单的培养系统，以与人胚胎发生相关的方式研究细胞分化的某些方面。在这里，我们回顾了用于获得和表征已建立和公认的人EC干细胞系TERA2.cl.SP12的策略。此外，我们证明了人EC干细胞作为早期发育模型的价值，并专注于胚胎外胚层细胞命运的确定。
There are few reliable experimental systems available to study the molecular mechanisms that govern human embryonic development. Embryonal carcinoma (EC) cells are pluripotent stem cells derived from teratocarcinomas and are considered the malignant counterparts of human embryonic stem (ES) cells. Several of the existing human EC stem cell lines provide robust and simple culture systems to study certain aspects of cellular differentiation in a manner pertinent to human embryogenesis. Here we review the strategies used to derive and characterize the established and recognized human EC stem cell line TERA2.cl.SP12. Furthermore, we demonstrate the value of human EC stem cells as a model of early development and focus on cell fate determination in the embryonic ectoderm.