Apoptosis during spontaneous luteolysis in the cyclic golden hamster: biochemical and morphological evidence.
Apoptosis during spontaneous luteolysis in the cyclic golden hamster: biochemical and morphological evidence.
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DOI：
10.1095/biolreprod58.1.255
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发表时间：
1998
影响因子：
3.6
通讯作者：
J. T. McCormack;M. Friederichs;S. D. Limback;G. S. Greenwald
中科院分区：
文献类型：
--
作者：
J. T. McCormack;M. Friederichs;S. D. Limback;G. S. Greenwald
周期性仓鼠的黄体 (CL) 在 4 天发情周期的第 2 天和第 3 天之间被破坏，因此只有一组存在（第 1 天 = 发情期，第 4 天 = 发情前期）。探讨了周期性仓鼠黄体细胞死亡归因于细胞凋亡的可能性。结构性黄体溶解的最早组织学迹象是在第 3 天 0600 小时检测到的，表现为一些分散的黄体细胞，显示出凋亡细胞的特征形态，并且有大量的中性粒细胞浸润。中性粒细胞流入和黄体凋亡的峰值分别在第3天1200小时和第3天2400小时达到峰值。因此，中性粒细胞的增加发生在黄体溶解主要发生之前。到第 3 天，即 2400 小时，CL 的重量已经缩小了三分之一，到第二天的第 1 天它们几乎消失了。细胞凋亡最终破坏了黄体内皮细胞、黄体细胞和中性粒细胞。对黄体细胞裂解物中低分子量 DNA 的电泳分析显示，从 1200 小时开始的第 3 天，寡核小体长度 DNA 片段具有明确的阶梯模式（细胞凋亡的特征）。在第 2 天收集的 CL 中未检测到该模式。将第 3 天在 0900-1200 小时收集的 CL 与 1500-1800 小时收集的 CL 进行比较表明，只有后者表现出核小体间裂解活性。第 3 天，即 1500 小时，证明 DNA 梯状排列所需的 CL 最少数量为 6 个。总之，寡核小体片段的电泳分离和组织学表明细胞凋亡发生在仓鼠周期第3天的自发黄体退化期间。直到功能性黄体溶解开始后几个小时，细胞凋亡的开始才明显。细胞凋亡在非常精确的时间安排内快速消除 CL，使循环仓鼠成为分析结构性黄体溶解所涉及因素的理想模型。
The corpora lutea (CL) of the cyclic hamster are destroyed between Days 2 and 3 of the 4-day estrous cycle so that only one set is ever present (Day 1 = estrus, Day 4 = proestrus). The possibility that luteal cell death in the cyclic hamster is attributable to apoptosis was explored. The earliest histological signs of structural luteolysis were detected at 0600 h of Day 3 as evidenced by a few scattered luteal cells displaying the characteristic morphology of apoptotic cells and by a massive infiltration of neutrophils. The peaks of neutrophil influx and luteal apoptosis were reached on Day 3, 1200 h, and Day 3, 2400 h, respectively. Thus, the increase in neutrophils occurs before the major onset of luteolysis. By Day 3, 2400 h, the CL had already shrunken one third by weight, and they virtually vanished by the next Day 1. Apoptosis ultimately destroyed luteal endothelial cells, luteal cells, and neutrophils. Electrophoretic analysis of low-molecular weight DNA in luteal cell lysates revealed a definite ladder pattern of oligonucleosomal-length DNA fragments--characteristic of apoptosis--on Day 3 beginning at 1200 h. The pattern was not detectable in CL collected on Day 2. Comparing Day 3 CL collected at 0900-1200 h with those at 1500-1800 h showed that only the latter group exhibited inter-nucleosomal cleavage activity. The minimal number of CL on Day 3, 1500 h, needed to demonstrate DNA laddering was six. In summary, the electrophoretic separation of oligonucleosomal fragments and histology indicated that apoptosis occurs during spontaneous luteal regression on Day 3 of the hamster cycle. The initiation of apoptosis is not apparent until several hours after the onset of functional luteolysis. The rapidity with which apoptosis eliminates the CL over a very precise time schedule makes the cyclic hamster an ideal model to analyze the factors involved in structural luteolysis.