Charged polypeptide vesicles with controllable diameter
Charged polypeptide vesicles with controllable diameter
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DOI：
10.1021/ja053557t
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发表时间：
2005-09-07
影响因子：
15
通讯作者：
Deming, TJ
中科院分区：
文献类型：
--
作者：
Holowka, EP;Pochan, DJ;Deming, TJ
我们报道了在水溶液中形成稳定的囊泡和胶束的带电两亲性嵌段共聚肽的制备和表征。具体地说，我们制备并研究了一系列聚(L-赖氨酸)-b-聚(L-亮氨酸)嵌段共聚肽KXLY(其中x从20到80，y从10到30个残基)以及聚(L-谷氨酸)-b-聚(L-亮氨酸)嵌段共聚肽E60L20。此外，囊泡组件表现出动态性质，表明膜具有很高的流动性。这一特性为囊泡提供了刺激响应特性，并允许使用基于脂质体的挤出技术来精细调整囊泡大小。囊泡挤压也提供了一种捕获溶质的直接方法，使囊泡成为很有前途的仿生胶囊。
We report the preparation and characterization of charged, amphiphilic block copolypeptides that form stable vesicles and micelles in aqueous solution. Specifically, we prepared and studied the aqueous self-assembly of a series Of poly(L-lysine)-b-poly(L-leucine) block copolypeptides, KxLy, where x ranged from 20 to 80 and y ranged from 10 to 30 residues, as well as the poly(L-glutamatic acid)-b-poly(L-leucine) block copolypeptide, E60L20. Furthermore, the vesicular assemblies show dynamic properties, indicating a high degree of membrane fluidity. This characteristic provides stimuli-responsive properties to the vesicles and allows fine adjustment of vesicle size using liposome-based extrusion techniques. Vesicle extrusion also provides a straightforward means to trap solutes, making the vesicles promising biomimetic encapsulants.