Identification and characterization of myophilin-like protein: a life stage and tissue-specific antigen of Clonorchis sinensis
Identification and characterization of myophilin-like protein: a life stage and tissue-specific antigen of Clonorchis sinensis
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肌亲蛋白样蛋白的鉴定和表征：华支睾吸虫的生命阶段和组织特异性抗原
DOI：
10.1007/s00436-012-2946-2
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发表时间：
2012-09-01
影响因子：
2
通讯作者：
Yu, Xinbing
中科院分区：
文献类型：
--
作者：
Huang, Yan;Li, Wenfang;Yu, Xinbing
由华支睾吸虫感染引起的华支睾吸虫病已成为我国重要的公共卫生问题。进一步了解了C.中华华支睾吸虫病防治新策略的开发。本研究利用已发表的C.在中华绒螯蟹中，我们鉴定了一个编码亲肌素样蛋白（Cs myophilin-like）的基因。该基因全长573 bp，编码190个氨基酸。Cs myophilin-like基因的DNA序列由4个外显子和3个内含子组成。通过绘制C. sinensis，注释了myophilin样基因的单拷贝。生物信息学分析表明，Cs myophilin like包含一个calponin同源结构域和三个肌动蛋白结合表面。它在成虫和囊蚴的生活阶段表达，但在卵中不表达。主要分布于成虫的口吸盘、咽部及囊蚴的吸盘等肌肉部位。Cs myophilin-like在成虫口吸盘和咽部的转录水平显著高于其它部位。获得了具有抗原性和免疫反应性的重组Cs亲肌素样蛋白。它可以用感染吸虫或绦虫的患者的血清探测。提示Cs myophilin-like蛋白除了调节平滑肌的收缩和虫体的运动外，还可能参与了宿主组织的侵袭。因此，重组Cs myophilin样蛋白在大肠杆菌中的稳定可溶性表达及免疫原性表明，Cs myophilin样蛋白可能成为华支睾吸虫病疫苗和药物的潜在候选靶点。
Clonorchiasis, caused by Clonorchis sinensis infection, has been an important public health problem in China. More messages about biology and pathogenicity of C. sinensis will be better for development of new strategies for clonorchiasis control. In the current study, using data from the published genomic and cDNA sequences for C. sinensis, we identify a gene encoding a myophilin-like protein (Cs myophilin-like). The cDNA sequence is 573 bp in length which encoded a protein of 190 amino acids. DNA sequence of Cs myophilin-like consists of four extrons and three introns. By mapping the draft genome of C. sinensis, single copy of myophilin-like gene is annotated. Bioinformatic analysis shows Cs myophilin-like comprises a calponin homology domain and three actin binding surfaces. It expresses at the life stage of adult worm and metacercaria but not in eggs. It intensively distributes in muscular locations such as oral sucker and pharynx of adult worm and sucker of metacercaria. The transcript level of Cs myophilin-like in oral sucker and pharynx is significantly higher than that in the rest of adult worm. Moreover, recombinant Cs myophilin-like is obtained and of antigenicity and immunoreactivity. It can be probed by sera from patients infected with trematode or cestode. Our data suggests that Cs myophilin-like may be involved in the host tissue invasion besides regulating the contraction of smooth muscle and movement of worm body. Taken together, the stable and soluble expression in Escherichia coli and immunogenicity of recombinant Cs myophilin-like proteins suggest that Cs myophilin-like may be a potential candidate for vaccine and drug target for clonorchiasis.