Existence of ATP-evoked ATP release system in smooth muscles.
Existence of ATP-evoked ATP release system in smooth muscles.
复制标题
平滑肌中存在 ATP 诱发的 ATP 释放系统。
DOI：
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发表时间：
1991
影响因子：
3.5
通讯作者：
T. Furukawa
中科院分区：
文献类型：
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作者：
T. Katsuragi;T. Tokunaga;S. Ogawa;O. Soejima;C. Sato;T. Furukawa
研究了稳定的ATP类似物α、β -亚甲基ATP （α, β - matp）和β、γ -亚甲基ATP （β, γ - matp）对豚鼠输精管和回肠纵肌ATP释放和收缩反应的影响。在这些平滑肌中，给药α、β -甲基ADP（10、30或100微米）产生ATP释放并伴有短暂的收缩，但α、β -亚甲基ADP（30或100微米）或腺苷（30微米）不能同时引起ATP释放和收缩。而输精管对α、β - matp（100微米）的ATP释放和收缩的峰值分别出现在注射后2 min和2.62 s左右。β, γ - matp（10或100微米）引起输精管释放ATP。300微米苏拉明（一种P2嘌呤受体拮抗剂）可有效抑制α、β - matp或β、γ - matp引起的ATP释放和收缩。相比之下，输精管中去甲肾上腺素的ATP释放和收缩反应以及回肠中去甲肾上腺素的收缩反应几乎不受这种拮抗剂的影响。在（30或100微米）下，韦曲定和乌阿拜分别引起回肠乙酰胆碱释放和输精管去甲肾上腺素释放。然而，即使在100微米的高浓度下，α、β - matp也不会引起乙酰胆碱或去甲肾上腺素的释放。这些发现表明，α、β - matp和β、γ - matp通过激活素胺敏感的P2x受体，从非神经元而主要是平滑肌部位激发ATP释放，这意味着存在“ATP诱发的ATP释放系统”。
Effects of stable ATP analogs such as alpha,beta-methylene ATP (alpha,beta-mATP) and beta,gamma-methylene ATP (beta,gamma-mATP) on ATP release and contractile response were evaluated in the vas deferens and ileal longitudinal muscles of guinea pig. In these smooth muscles, administration of alpha,beta-mATP (10, 30 or 100 microM) produced an ATP release accompanied by a transient contraction, but alpha,beta-methylene ADP (30 or 100 microM) or adenosine (30 microM) failed to elicit both the ATP release and the contraction. However, the peak responses of ATP release and contraction to alpha,beta-mATP (100 microM) in the vas deferens appeared around 2 min and 2.62 sec, respectively, after the injection of the drug. Beta,gamma-mATP (10 or 100 microM) caused an ATP release from the vas deferens. The ATP release as well as the contraction evoked by alpha,beta-mATP or beta,gamma-mATP were effectively inhibited by 300 microM suramin, a P2 purinoceptor antagonist. By contrast, ATP release and contractile response to norepinephrine in the vas deferens and those to bethanechol in the ileum were virtually unaffected by this antagonist. Veratridine and ouabain at (30 or 100 microM) caused markedly acetylcholine release from the ileum and norepinephrine release from the vas deferens, respectively. However, alpha,beta-mATP, even in a high concentration of 100 microM, did not elicit any release of acetylcholine or norepinephrine. These findings suggest that alpha,beta-mATP and probably beta,gamma-mATP evoke ATP release from not neuronal but mainly smooth muscular sites by activating suramin-sensitive P2x receptors, implying that "ATP-evoked ATP release system" exists.