Rotavirus-neutralizing antibodies inhibit virus binding to integrins alpha 2 beta 1 and alpha 4 beta 1.
Rotavirus-neutralizing antibodies inhibit virus binding to integrins alpha 2 beta 1 and alpha 4 beta 1.
复制标题
轮状病毒中和抗体可抑制病毒与整合素 α2β1 和 α4β1 的结合。
作者：
F. Fleming;K. Graham;K. Taniguchi;Y. Takada;B. Coulson
轮状病毒外衣壳蛋白VP 5（*）、VP 8（*）和VP 7引发中和性、保护性抗体。α 2 β 1整联蛋白是轮状病毒的细胞受体，与VP 5结合（*）。一些轮状病毒也识别α 4 β 1整联蛋白。在这项研究中，轮状病毒的抗体对病毒结合重组α 2 β 1和α 4 β 1在K562细胞上表达的影响进行了测定。所有测试的针对VP 5（*）的中和单克隆抗体（YO-2C 2、2G 4、1A 10）和两种针对VP 7的中和单克隆抗体（RV-3：2、RV-4：2）抑制轮状病毒与α 2 β 1的结合。通过2G 4和针对VP 7的中和抗体F45：2降低轮状病毒与α 4 β 1的结合。然而，针对VP 8（*）（RV-5：2）和针对VP 7（RV-3：1）的中和抗体不影响轮状病毒与这些整联蛋白的结合。病毒-细胞结合不受轮状病毒内衣壳蛋白VP 6的非中和抗体RVA的影响。从两名患有严重轮状病毒胃肠炎住院的儿童中收集的具有中和活性的感染血清抑制了人轮状病毒株Wa与这些整合素的附着。阴性参考血清不影响轮状病毒细胞附着。由于轮状病毒与α 2 β 1和α 4 β 1的结合被VP 5（*）和VP 7中和抗体以及轮状病毒病儿童血清抑制，因此轮状病毒识别这些整合素可能对宿主感染很重要。
Rotavirus outer capsid proteins VP5(*), VP8(*) and VP7 elicit neutralizing, protective antibodies. The alpha 2 beta 1 integrin is a cellular receptor for rotavirus that is bound by VP5(*). Some rotaviruses also recognize the alpha 4 beta 1 integrin. In this study, the effects of antibodies to rotavirus on virus binding to recombinant alpha 2 beta 1 and alpha 4 beta 1 expressed on K562 cells were determined. All neutralizing monoclonal antibodies to VP5(*) tested (YO-2C2, 2G4, 1A10) and two to VP7 (RV-3:2, RV-4:2) inhibited rotavirus binding to alpha 2 beta 1. Rotavirus binding to alpha 4 beta 1 was reduced by 2G4 and neutralizing antibody F45:2, directed to VP7. However, a neutralizing antibody to VP8(*) (RV-5:2) and one to VP7 (RV-3:1) did not affect rotavirus binding to these integrins. Virus-cell binding was unaffected by non-neutralizing antibody RVA to the rotavirus inner capsid protein VP6. The attachment of human rotavirus strain Wa to these integrins was inhibited by infection sera with neutralizing activity collected from two children hospitalised with severe rotavirus gastroenteritis. A negative reference serum did not affect rotavirus-cell attachment. As the binding of rotaviruses to alpha 2 beta 1 and alpha 4 beta 1 is inhibited by neutralizing antibodies to VP5(*) and VP7, and serum from children with rotavirus disease, rotavirus recognition of these integrins may be important for host infection.