Evidence for the monophyletic evolution of benzylisoquinoline alkaloid biosynthesis in angiosperms
Evidence for the monophyletic evolution of benzylisoquinoline alkaloid biosynthesis in angiosperms
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DOI：
10.1016/j.phytochem.2005.04.029
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发表时间：
2005-06-01
期刊：
影响因子：
3.8
通讯作者：
Facchini, PJ
中科院分区：
文献类型：
--
作者：
Liscombe, DK;MacLeod, BP;Facchini, PJ
苄基异喹啉生物碱(BIAS)由2500多种不同的结构组成，主要局限于毛滴目和真核生物碱。然而，偏见也出现在十字花科、樟科、山茱莉科和荷花科中，并零星地出现在胡椒目的各处。其中一些生物碱在植物抵御食草动物和病原体方面发挥着重要作用--因此，BIA生物合成的能力有望在某些植物的繁殖适合性中发挥重要作用。采用生物化学和分子系统学方法研究了被子植物BIA生物合成的进化过程。比较了90种不同植物中(S)去甲黄嘌呤合成酶(NCS；EC 4.2.1.78)活性的出现情况与分子系统发育上的倾向性分布。这些结果支持BIA生物合成的单系起源早于真雄蕊的出现。对NCS、黄连素桥酶和几种O-甲基转移酶的系统发育分析表明，在未知积累此类生物碱的被子植物中存在BIA生物合成的潜在分子指纹。在Ranunculales和Eumagnoliides之外的偏向的出现是有限的，这表明需要一个高度专业化但在进化上不稳定的细胞平台来适应或重新激活在不同分类群中的途径。罂粟NCS基因的克隆及其功能研究也有报道说。致病相关(PR)10和Bet V I主要变应原蛋白与NCS有同源性，但重组多肽缺乏NCS活性。(C)2005爱思唯尔有限公司。保留所有权利。
Benzylisoquinoline alkaloids (BIAs) consist of more than 2500 diverse structures largely restricted to the order Ranunculales and the eumagnoliids. However, BIAs also occur in the Rutaceae, Lauraceac, Cornaceae and Nelumbonaceae, and sporadically throughout the order Piperales. Several of these alkaloids function in the defense of plants against herbivores and pathogens - thus, the capacity for BIA biosynthesis is expected to play an important role in the reproductive fitness of certain plants. Biochemical and molecular phylogenetic approaches were used to investigate the evolution of BIA biosynthesis in basal angiosperms. The occurrence of (S)-norcoclaurine synthase (NCS; EC 4.2.1.78) activity in 90 diverse plant species was compared to the distribution of BIAs superimposed onto a molecular phylogeny. These results support the monophyletic origin of BIA biosynthesis prior to the emergence of the eudicots. Phylogenetic analyses of NCS, berberine bridge enzyme and several O-methyltransferases suggest a latent molecular fingerprint for BIA biosynthesis in angiosperms not known to accumulate such alkaloids. The limited occurrence of BIAs outside the Ranunculales and eumagnoliids suggests the requirement for a highly specialized, yet evolutionarily unstable cellular platform to accommodate or reactivate the pathway in divergent taxa. The molecular cloning and functional characterization of NCS from opium poppy (Papaver somniferum L.) is also reported. Pathogenesis-related (PR)10 and Bet v I major allergen proteins share homology with NCS, but recombinant polypeptides were devoid of NCS activity. (c) 2005 Elsevier Ltd. All rights reserved.