Measles virus-specific human T cell clones. Characterization of specificity and function of CD4+ helper/cytotoxic and CD8+ cytotoxic T cell clones.
Measles virus-specific human T cell clones. Characterization of specificity and function of CD4+ helper/cytotoxic and CD8+ cytotoxic T cell clones.
复制标题
麻疹病毒特异性人类 T 细胞克隆。
作者：
R. Binnendijk;M. Poelen;P. Vries;H. O. Voorma;A. Osterhaus;F. Uytdehaag
对麻疹病毒（MV）血清阳性的健康成人个体的PBMC检测其增殖为UV灭活的MV（UV-MV）或自体MV感染的EBV转化的B细胞系（EBV-BC）的能力。MV特异性T细胞反应中观察到11 15个捐助者测试（刺激指数大于2），当最佳剂量的UV-MV用于增殖试验。通过使用UV-MV或MV感染的自体EBV-BC作为APC，从响应MV的三个供体的PBMC产生T细胞克隆。用UV-MV刺激仅产生CD 3 + CD 4 + CD 8-MV特异性T细胞，而用MV感染的EBV-BC刺激PBMC后，获得CD 3 + CD 4 + CD 8-和CD 3 + CD 4-CD 8 + MV特异性T细胞克隆。在19个CD 4 + T细胞克隆中，7个克隆与纯化的融合蛋白特异性反应，1个克隆与纯化的血凝素蛋白特异性反应。7个克隆增殖响应MV的内部蛋白。三个克隆反应，整个病毒，但不是一个纯化的蛋白质，而一个克隆似乎认识到一个以上的多肽。当用MV感染的EBV-BC代替UV-MV和PBMC作为APC时，由UV-MV体外刺激PBMC产生的一些T细胞克隆不能识别MV Ag。CD 3 + CD 4 + CD 8- T细胞克隆可识别与HLA Ⅱ类抗原（HLA-DQ或-DR）相关的MV，且大多数克隆对MV感染的EBV-BC表现出CTL活性。迄今为止测试的所有CD 4 + HLA II类限制性CTL克隆都能够辅助B淋巴细胞产生MV特异性抗体。CD 4-CD 8 + T细胞克隆MARO 1识别与HLA I类分子相关的MV，并显示出对MV感染的EBV-BC的细胞毒性活性。
PBMC from healthy adult individuals seropositive for measles virus (MV) were tested for their capacity to proliferate to UV-inactivated MV (UV-MV) or to autologous MV-infected EBV-transformed B cell lines (EBV-BC). MV-specific T cell responses were observed in 11 of 15 donors tested (stimulation index greater than 2), when optimal doses of UV-MV were used in proliferative assays. T cell clones were generated from PBMC of three donors responding to MV, by using either UV-MV or MV-infected autologous EBV-BC as APC. Stimulation with UV-MV generated exclusively CD3+ CD4+ CD8- MV-specific T cells, whereas after stimulation of PBMC with MV-infected EBV-BC, both CD3+ CD4+ CD8- and CD3+ CD4- CD8+ MV-specific T cell clones were obtained. Of 19 CD4+ T cell clones tested so far, 7 clones reacted specifically with purified fusion protein and 1 with purified hemagglutinin protein. Seven clones proliferated in response to the internal proteins of MV. Three clones reacted to whole virus but not to one of the purified proteins, whereas one clone seemed to recognize more than one polypeptide. Some of the T cell clones, generated from in vitro stimulation of PBMC with UV-MV, failed to recognize MV Ag when MV-infected EBV-BC were used as APC instead of UV-MV and PBMC. CD3+ CD4+ CD8- T cell clones recognized MV in association with HLA class II Ag (HLA-DQ or -DR), and most of them displayed CTL activity to autologous MV-infected EBV-BC. All CD4+ HLA class II-restricted CTL clones thus far tested were capable of assisting B lymphocytes for the production of MV-specific antibody. The CD4- CD8+ T cell clone MARO 1 recognized MV in association with HLA class I molecules and displayed cytotoxic activity toward MV-infected EBV-BC.