Determination of inflammatory biomarkers by immunoaffinity capillary electrophoresis.
Determination of inflammatory biomarkers by immunoaffinity capillary electrophoresis.
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DOI：
10.1016/j.ddtec.2006.03.009
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发表时间：
2006-01-01
期刊：
影响因子：
--
通讯作者：
Phillips, Terry M
中科院分区：
文献类型：
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作者：
Guzman, Norberto A;Stubbs, R John;Phillips, Terry M
迫切需要仪器和方法的进步，以实现对生物体液、组织和细胞中各种浓度的多种物质的快速、同步和灵敏测定。免疫亲和毛细管电泳在生命科学中的应用已经对许多用于诊断和监测疾病预后的生物标志物的定量产生了影响。本综述解释了免疫亲和毛细管电泳（高选择性抗体捕获剂与毛细管电泳高分辨率的结合）如何提供高度特异性的检测，从而实现复杂生物基质中感兴趣分析物的选择性分离、浓缩、分离和定量。除了技术讨论之外，还将介绍一些临床和制药相关的应用：
Advances in instrumentation and methodologies are urgently needed to achieve, rapid, simultaneous and sensitive determination of multiple substances found at a wide range of concentrations in biological fluids, tissues and cells. The application of immunoaffinity capillary electrophoresis in life sciences is already having an impact on the quantification of many biomarkers for diagnosis and monitoring the prognosis of diseases. This review explains how immunoaffinity capillary electrophoresis, the combination of highly selective antibody capture agents with the high resolving power of capillary electrophoresis, can provide highly specific assays leading to the selective isolation, concentration, separation and quantification of analytes of interest in complex biological matrices. In addition to a discussion of the technology, some applications of clinical and pharmaceutical relevance will be presented.: