The kic1 kinase of schizosaccharomyces pombe is a CLK/STY orthologue that regulates cell-cell separation.
The kic1 kinase of schizosaccharomyces pombe is a CLK/STY orthologue that regulates cell-cell separation.
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DOI：
10.1016/s0014-4827(02)00022-8
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发表时间：
2003-02
影响因子：
3.7
通讯作者：
Zhaohua Tang;Linda L Mandel;S. Yean;Cindy X. Lin;T. Chen;M. Yanagida;R. Lin
中科院分区：
文献类型：
--
作者：
Zhaohua Tang;Linda L Mandel;S. Yean;Cindy X. Lin;T. Chen;M. Yanagida;R. Lin
CLK/STY激酶是一个涉及细胞生长和分化调控的双特异性蛋白激酶家族。该家族中的一些激酶被证明可以磷酸化富含丝氨酸精氨酸的剪接因子并调节mrna前剪接。然而，CLK/STY调控细胞生长、分化和发育的实际细胞机制尚不清楚。在这里，我们发现在裂糖酵母中存在一个功能保守的CLK/STY激酶，这个同源物被称为Kic1，调节细胞表面和隔膜的形成以及细胞质分裂的后期步骤。Kic1蛋白在体内被修饰，可能是通过磷酸化，这表明它可能参与了控制级联反应。此外，kic1+与编码相关sr特异性蛋白激酶的dsk1+一起构成了细胞生长的关键体内功能。该结果首次在体内证明了CLK/STY家族在从裂变酵母到哺乳动物的进化过程中功能的保存。此外，由于细胞分裂和细胞间相互作用是生物分化和发育的基础，本研究揭示的kic1+的新细胞作用为理解其在高等真核生物中的对应物提供了线索。
The CLK/STY kinases are a family of dual-specificity protein kinases implicated in the regulation of cellular growth and differentiation. Some of the kinases in the family are shown to phosphorylate serine–arginine-rich splicing factors and to regulate pre-mRNA splicing. However, the actual cellular mechanism that regulates cell growth, differentiation, and development by CLK/STY remains unclear. Here we show that a functionally conserved CLK/STY kinase exists in Schizosaccharomyces pombe, and this orthologue, called Kic1, regulates the cell surface and septum formation as well as a late step in cytokinesis. The Kic1 protein is modified in vivo, likely by phosphorylation, suggesting that it can be involved in a control cascade. In addition, kic1+together with dsk1+, which encodes a related SR-specific protein kinase, constitutes a critical in vivo function for cell growth. The results provide the first in vivo evidence for the functional conservation of the CLK/STY family through evolution from fission yeast to mammals. Furthermore, since cell division and cell–cell interaction are fundamental for the differentiation and development of an organism, the novel cellular role of kic1+revealed from this study offers a clue to the understanding of its counterparts in higher eukaryotes.