Screening of CBL genes in pigeon pea with focus on the functional analysis of CBL4 in abiotic stress tolerance and flavonoid biosynthesis
Screening of CBL genes in pigeon pea with focus on the functional analysis of CBL4 in abiotic stress tolerance and flavonoid biosynthesis
复制标题
木豆CBL基因筛选，重点分析CBL4在非生物胁迫耐受和类黄酮生物合成中的功能分析
DOI：
10.1016/j.envexpbot.2020.104102
复制
发表时间：
2020
影响因子：
5.7
通讯作者：
Fu Yujie
中科院分区：
文献类型：
--
作者：
Song Zhihua;Dong Biying;Yang Qing;Niu Lili;Li Hanghang;Cao Hongyan;Amin Rohul;Meng Dong;Fu Yujie
木豆是世界上唯一的木本豆科植物，也是药食同源作物。与此同时，介导多种非生物胁迫反应的钙调神经磷酸酶 B 样 (CBL) 蛋白在很大程度上尚不清楚。在此，我们对木豆中的CBL家族进行了生物信息学分析，初步验证了CcCBL4在抗逆性和次生代谢合成方面的功能。 CBL家族基因响应4℃、42℃、NaCl和干旱胁迫，我们观察到CcCBL4-OE（过表达）植株的叶绿素、相对水份、脯氨酸和可溶性糖在胁迫后显着高于CK（空载体）植株，且细胞膜的完整性更好。检测到7种次生代谢物，发现OE中的对香豆酸含量是CK植物的1.4倍，但芹菜素和紫檀芪减少。并且这些通路中基因的表达也被证实发生了变化。总之，我们的工作揭示了CcCBL蛋白、抗逆性和类黄酮代谢之间的潜在调控机制，有利于增强木豆对环境的适应性。
Pigeon pea, the only woody legume in the world,is also a medicinal-food homogenous crop. At the same time, the calcineurin B-like (CBL) proteins that mediate a variety of abiotic stress responses are largely unknown. Here, we performed a bioinformatics analysis of the CBL family in pigeon pea, and initially verified the function of CcCBL4 in stress resistance and secondary metabolic synthesis. CBL family genes respond to 4 °C, 42 °C, NaCl, and drought stresses, and we observed the chlorophyll, relative water, proline and soluble sugar in CcCBL4-OE (over-expression) were significantly higher than CK (empty vectors) plants after stresses, and the integrity of the cell membrane was better. Seven secondary metabolites were detected, and we found that the p-coumaric acid in OE was 1.4-fold that in CK plants, but the apigenin and pterostilbene were reduced. And the expression of genes in these pathways was also confirmed changes. Altogether, our work revealed a potential regulation mechanism between CcCBL proteins, stress resistance and flavonoid metabolism, which is conducive to enhance the adaptability of pigeon pea to the environment.