Leaching alleviates phenol-mediated root rot in Panax notoginseng by modifying the soil microbiota
Leaching alleviates phenol-mediated root rot in Panax notoginseng by modifying the soil microbiota
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DOI：
10.1007/s11104-021-05136-z
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发表时间：
2021-09
期刊：
影响因子：
4.9
通讯作者：
Yijie Zhang;Tian-lin Li;C. Ye;Renchuang Lu;Yixiang Liu;Huichuan Huang;Xiahong He;Min Yang
中科院分区：
文献类型：
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作者：
Yijie Zhang;Tian-lin Li;C. Ye;Renchuang Lu;Yixiang Liu;Huichuan Huang;Xiahong He;Min Yang
土壤中树木化感物质的过量积累会影响三七的生长，并引起根腐病。许多植物分泌的酚类物质是水溶性物质，可通过淋溶而被耗尽。然而，目前对杉木土壤酚类淋溶对三七根腐病的影响还缺乏全面的认识。采用5种不同强度的酚类污染土壤淋洗，研究其对三七根腐病的影响。分析了根腐病与土壤化学性质、酚含量变化及根际微生物的关系。随着淋溶强度的增加，土壤中酚类物质含量和根腐病发生率显著降低。降低土壤酚含量可以通过改变土壤微生物群落来减轻根腐病。网络分析表明，根腐病发病率与富含对羟基苯甲酸的病原真菌Ilyonectria呈正相关，而与鞘氨醇单胞菌属（Sphingomonas）、溶杆菌属（Lysobacter）、Massilia和伯克霍尔德氏菌属（Burkholderia）等对羟基苯甲酸（HA）、阿魏酸（FA）和香草酸（VA）抑制细菌呈负相关。培养实验证实，HA、FA和VA单独使用以及5种酚类物质的混合物随浓度的增加均能促进I.破坏素，但抑制细菌（伯克霍尔德氏菌属和溶杆菌属）的生长和生物膜形成。此外，伯克霍尔德氏菌分离株对I. destructans。淋溶可缓解酚类物质对病原真菌的刺激，减轻酚类抑制菌的压力，从而降低根腐病的发病率。
Overaccumulation of allelochemicals in soil from trees has a negative effect on the growth of Panax notoginseng and cause root rot. Many plant secreted phenols are water-soluble substances that can be depleted by leaching. However, comprehensive understanding of the effect of leaching of phenols in Chinese fir soil on P. notoginseng root rot disease is still lacking. Soil with high level of phenols was leached with five intensities and their effect on the root rot of P. notoginseng was assessed. Then, the relationship of root rot with changes in soil chemical properties and phenol content as well as rhizospheric microorganisms was analyzed. With increasing leaching intensity, the levels of phenols in soils and the incidence of root rot decreased significantly. Decreased phenol content could alleviate root rot by modifying the soil microbial community. Network analysis showed that root rot incidence was positively correlated with the p-hydroxybenzoic acid-enriched pathogenic fungus Ilyonectria but negatively correlated with p-hydroxybenzoic acid (HA)-, ferulic acid (FA)- or vanillic acid (VA)-suppressed bacteria, including Sphingomonas, Lysobacter, Massilia and Burkholderia. Culture experiments confirmed that individual HA, FA and VA as well as a mixture of the five phenols with increasing concentrations could stimulate the growth and sporulation of I. destructans but suppress the growth and biofilm formation of bacteria (Burkholderia and Lysobacter). Moreover, Burkholderia isolates showed antagonistic activity against the growth of I. destructans. Leaching could relieve the stimulation of pathogenic fungi by phenols and reduce the pressure on phenolic-suppressed bacteria, thereby reducing the incidence of root rot disease.