Hepatic Stellate Cells Contribute to the Tumor Malignancy of Hepatocellular Carcinoma Through the IL-6 Pathway
Hepatic Stellate Cells Contribute to the Tumor Malignancy of Hepatocellular Carcinoma Through the IL-6 Pathway
复制标题
DOI：
10.21873/anticanres.14005
复制
发表时间：
2020-02-01
影响因子：
2
通讯作者：
Shimada, Mitsuo
中科院分区：
文献类型：
--
作者：
Iwahasi, Shuichi;Rui, Feng;Shimada, Mitsuo
背景/目的：肝星状细胞（hepatic stellate cells，HSCs）与肿瘤的发生发展有关。本研究的目的是探讨肝星状细胞的作用和IL-6/Stat 3通路在肝细胞癌（HCC）进展中的作用。材料与方法：将肝癌细胞与肝星状细胞共培养。检测癌细胞的存活率和迁移能力。评价癌细胞的上皮-间质转化（EMT）标志物（E-cadherin）、干细胞标志物（CD 44）和p-信号转导子和转录激活子3（p-STAT 3）。最后，进行白细胞介素-6（IL-6）中和。结果：肝细胞癌与肝星状细胞共培养可提高癌细胞的存活率和迁移能力。癌细胞的EMT和干细胞性随着HSC的增加而增加。在IL-6中和后，磷酸化-STAT 3活化、癌细胞活力和迁移以及EMT和癌细胞的干细胞性降低。结论：HSCs通过IL-6/STAT 3途径促进HCC的进展。
Background/Aim: The hepatic stellate cells (HSCs) have relationship to cancer progression. The aim of this study is to investigate the effect of HSCs and the role of IL-6/Stat3 pathway on hepatocellular carcinoma (HCC) progression. Materials and Methods: HCCs were co-cultured with HSCs. The viability and migration ability of cancer cells were detected. Epithelial-mesenchymal transition (EMT) marker (E-cadherin), stem cell marker (CD44) and p-signal transducer and activator of transcription 3 (p-STAT3) of cancer cells were evaluated. Finally, interleukin-6 (IL-6) neutralization was performed. Results: Co-culture of HCCs with HSCs increased cancer cell viability and migration ability. EMT and stemness of cancer cells increased with HSCs. Following IL-6 neutralization, phospho-STAT3 activation, cancer cell viability and migration, as well as EMT, and stemness of cancer cells decreased. Conclusion: HSCs promoted HCC progression through the IL-6/STAT3 pathway.