A METHOD FOR THE ISOLATION OF LONGEVITY MUTANTS IN THE NEMATODE CAENORHABDITIS-ELEGANS AND INITIAL RESULTS
A METHOD FOR THE ISOLATION OF LONGEVITY MUTANTS IN THE NEMATODE CAENORHABDITIS-ELEGANS AND INITIAL RESULTS
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DOI：
10.1016/0047-6374(83)90082-9
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发表时间：
1983-01-01
影响因子：
5.3
通讯作者：
KLASS, MR
中科院分区：
文献类型：
--
作者：
KLASS, MR
自由生活的线虫秀丽隐杆线虫被用作衰老研究的遗传可操作实验系统。利用具有正常寿命的温度敏感无菌菌株，描述了一种分离寿命显着增加的突变菌株的方法。分离出八个突变株，每个突变株的寿命都延长了。两个突变株是自发的多尔形成者，这也是它们寿命延长的原因。另一种是趋化性缺陷，导致食物摄入量减少，这可能是其寿命延长的原因。五个突变体遭受不同程度的瘫痪，影响了他们的咽部泵送和食物摄入的速度。这些突变体食物摄入率的降低与其寿命的延长之间存在高度相关性，这表明寿命的延长很可能是由于热量摄入的减少。显然，特定的寿命基因极其罕见，或者寿命是通过多基因方式控制的。
The free-living nematode C. elegans is used as a genetically manipulable experimental system for the study of aging. Utilizing a temperature-sensitive sterile strain with a normal life span, a method is described for the isolation of mutant strains with significantly increased life spans. Eight mutant strains were isolated each having increased life spans. Two mutant strains were spontaneous dauer formers, accounting for their increased longevity. Another was chemotaxis-defective, causing reduced food intake which could account for its increased life span. Five mutants suffered from varying degrees of paralysis affecting their rate of pharyngeal pumping and food ingestion. The high correlation of the decreased rate of food ingestion of these mutants with their increased longevity is interpreted as indicating that the increased longevity is most likely due to reduced caloric intake. Apparently, specific life span genes are extremely rare or, alternatively, life span is controlled in a polygenic fashion.