Preparing synaptoneurosomes from adult mouse forebrain.
Preparing synaptoneurosomes from adult mouse forebrain.
复制标题
从成年小鼠前脑制备突触神经体。
DOI：
10.1007/978-1-62703-083-0_14
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Smalheiser,NeilR
中科院分区：
文献类型：
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作者：
Lugli,Giovanni;Smalheiser,NeilR
Many neuroscience studies involve subcellular fractionation to produce isolated or enriched synaptic fractions. Synaptosomes are prepared by flotation of synaptic membranes on sucrose or Percoll gradients. Alternatively, synaptoneurosomes are prepared by filtration of tissue homogenate through a series of filters to obtain a fraction that is enriched in pinched-off dendritic spines. Whereas the protocol for making synaptosomes is reasonably well standardized and well described in the literature, there is (to our knowledge) no detailed lab protocol for making synaptoneurosomes. Here, we give the methods used in our laboratory to produce synaptoneurosomes that are suitable for studying RNAs and proteins.
