Characterization, Expression and Function Analysis of DAX1 Gene of Scallop (Chlamys farreri Jones and Preston 1904) During Its Gametogenesis
Characterization, Expression and Function Analysis of DAX1 Gene of Scallop (Chlamys farreri Jones and Preston 1904) During Its Gametogenesis
复制标题
DOI：
10.1007/s11802-014-2299-9
复制
发表时间：
2014-08-01
影响因子：
1.6
通讯作者：
Zhang Zhifeng
中科院分区：
文献类型：
--
作者：
Li Hailong;Liu Jianguo;Zhang Zhifeng
DAX1 是核受体超家族的成员，在多种脊椎动物的性别决定和性腺分化中具有功能。然而，关于无脊椎动物 DAX1 的信息很少。在这里，我们克隆了扇贝（Chlamys farreri Jones and Preston 1904）dax1的同源物Cf-dax1，并确定了其在mRNA和蛋白质水平上的表达特征。 Cf-dax1 cDNA序列全长2093 bp，其中开放阅读框(ORF)长1404 bp，编码467个氨基酸。与脊椎动物不同的是，在 Cf-DAX1 的假定 DNA 结合区中没有发现保守的 LXXLL 相关基序。荧光原位杂交显示Cf-dax1位于一对亚端着丝粒染色体的短臂上。半定量RT-PCR组织分布分析显示，Cf-dax1在成年扇贝组织中广泛表达，其中内收肌中表达量最高，外套膜、鳃和睾丸中表达量中等，肾脏、卵巢和肝胰腺中表达量较低。实时荧光定量PCR结果显示，Cf-dax1的表达量显着升高（P
DAX1, a member of nuclear receptor superfamily, has a function in the sex determination and gonadal differentiation of several vertebrate species. However, little information about DAX1 of invertebrates is available. Here we cloned a homolog of scallop (Chlamys farreri Jones and Preston 1904) dax1, Cf-dax1, and determined its expression characteristics at mRNA and protein levels. The cDNA sequence of Cf-dax1 was 2093 bp in length, including 1404 bp open reading frame (ORF) encoding 467 amino acids. Unlike those of vertebrates, no conserved LXXLL-related motif was found in the putative DNA binding region of Cf-DAX1. Fluorescence in situ hybridization showed that Cf-dax1 located on the short arm of a pair of subtelocentric chromosomes. Tissue distribution analysis using semi-quantitative RT-PCR revealed that Cf-dax1 expressed widely in adult scallop tissues, with the highest expression level found in adductor muscle, moderate level in mantle, gill and testis, and low level in kidney, ovary and hepatopancreas. The result of quantitative real-time PCR indicated that the expression of Cf-dax1 was significantly higher (P