Regulating bone formation via controlled scaffold degradation
Regulating bone formation via controlled scaffold degradation
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DOI：
10.1177/154405910308201111
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发表时间：
2003-11-01
影响因子：
7.6
通讯作者：
Mooney, DJ
中科院分区：
文献类型：
--
作者：
Alsberg, E;Kong, HJ;Mooney, DJ
人们普遍认为，将用作细胞移植载体的聚合物的降解速率与发育组织的生长速率相结合，将提高其数量或质量。为了验证这一假设，我们通过伽马射线照射高分子量的藻酸盐，得到了具有不同分子量和结构的聚合物，从而开发出了一系列具有不同降解速率的藻酸盐水凝胶。通过植入物的回收速率、质量和弹性模量来衡量，聚合物链尺寸的减小会提高其在体内的降解速率。然后，我们使用快速和缓慢降解的藻酸盐（用含RGD的肽进行共价修饰以控制细胞行为）来研究生物降解速率对体内骨组织发育的影响。降解速度更快的凝胶使骨形成的程度和质量显著提高。这些结果表明，生物材料的可降解性是通过细胞移植实现最佳组织再生的关键设计标准。
It is widely assumed that coupling the degradation rate of polymers used as cell transplantation carriers to the growth rate of the developing tissue will improve its quantity or quality. To test this hypothesis, we developed alginate hydrogels with a range of degradation rates by gamma-irradiating high-molecular-weight alginate to yield polymers of various molecular weights and structures. Decreasing the size of the polymer chains increased the degradation rate in vivo, as measured by implant retrieval rates, masses, and elastic moduli. Rapidly and slowly degrading alginates, covalently modified with RGD-containing peptides to control cell behavior, were then used to investigate the effect of biodegradation rate on bone tissue development in vivo. The more rapidly degrading gels led to dramatic increases in the extent and quality of bone formation. These results indicate that biomaterial degradability is a critical design criterion for achieving optimal tissue regeneration with cell transplantation.