STIMULATION AND INHIBITION OF ADENYLYL CYCLASES MEDIATED BY DISTINCT REGULATORY PROTEINS
STIMULATION AND INHIBITION OF ADENYLYL CYCLASES MEDIATED BY DISTINCT REGULATORY PROTEINS
复制标题
DOI：
10.1038/302706a0
复制
发表时间：
1983-01-01
期刊：
影响因子：
64.8
通讯作者：
BIRNBAUMER, L
中科院分区：
文献类型：
--
作者：
HILDEBRANDT, JD;SEKURA, RD;BIRNBAUMER, L
Adenylyl cyclases are under positive and negative control by guanine nucleotides and hormones1–3. Stimulatory responses are mediated by a guanine nucleotide- and Mg-binding regulatory component4–6(Ns), a protein that has been purified to homogeneity7–9. Inhibitory responses have been hypothesized1–3,10,11to be mediated by an analogous regulatory component (Ni) distinct from Ns, but definitive proof for this is lacking and these effects may result from modulation of Ns activity. Recently,Bordetella pertussistoxin has been shown12to ADP-ribosylate a peptide that is not part of Ns, and this coincides with attenuation of hormonal inhibition of adenylyl cyclase12–17. We show here thatcyc−S49 cells contain a substrate for ADP-ribosylation by pertussis toxin and that the toxin alters GTP dependent inhibition ofcyc−adenyl cyclase activity18. Ascyc−S49 cells do not contain Nsby several criteria5,19–24, we conclude that Niis a distinct and separate regulatory component of adenylyl cyclase.
