Effect of cordycepin (3'-deoxyadenosine) on hematogenic lung metastatic model mice.
Effect of cordycepin (3'-deoxyadenosine) on hematogenic lung metastatic model mice.
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DOI：
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发表时间：
2005
期刊：
影响因子：
2.3
通讯作者：
Kazuki Nakamura;Keiko Konoha;N. Yoshikawa;Y. Yamaguchi;S. Kagota;K. Shinozuka;M. Kunitomo
中科院分区：
文献类型：
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作者：
Kazuki Nakamura;Keiko Konoha;N. Yoshikawa;Y. Yamaguchi;S. Kagota;K. Shinozuka;M. Kunitomo
我们研究了虫草素（3′-脱氧腺苷）对血源性转移小鼠模型静脉注射B16-BL6黑色素瘤细胞的抗转移作用。在肿瘤注射后15天，暴露于不同浓度的虫草素（0.3、1和3微克/毫升）3小时，剂量依赖性地减少了肺中形成的结节数量。为了阐明这种抗转移作用的机制，我们利用体外化学侵袭室检测了虫草素对B16-BL6细胞侵袭性的影响。虫草素（3 μ g/ml）预处理3小时后，B16-BL6细胞侵袭性明显降低。在相同条件下，虫草素浓度达到3 μ g/ml时，对B16-BL6细胞的生长曲线没有影响。这些结果表明，虫草素发挥抗转移作用，部分是通过抑制小鼠黑色素瘤细胞的侵袭性。
We investigated the anti-metastatic effect of cordycepin (3'-deoxyadenosine) on a hematogenic metastatic mouse model which was intravenously injected with B16-BL6 melanoma cells. A 3-hour exposure to various concentrations of cordycepin (0.3, 1 and 3 microg/ml) dose-dependently reduced the number of nodules formed in lung at 15 days after the tumor injection. To elucidate the mechanism of this anti-metastatic effect, we examined the effect of cordycepin on the invasiveness of B16-BL6 cells using a chemo-invasion chamber in vitro. The B16-BL6 cells pretreated with cordycepin (3 microg/ml) for 3 hours showed a significant decrease in invasiveness. Under the same conditions, however, cordycepin did not influence the growth curve of B16-BL6 cells at concentrations up to 3 microg/ml. These results suggest that cordycepin exerts an anti-metastatic action, in part, by inhibiting the invasiveness of mouse melanoma cells.