Unique temperature dependence in the adduct formation between FMN and cysteine S-H group in the LOV2 domain of Adiantum phytochrome3
Unique temperature dependence in the adduct formation between FMN and cysteine S-H group in the LOV2 domain of Adiantum phytochrome3
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DOI：
10.1016/j.cplett.2005.05.049
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发表时间：
2005-07-10
影响因子：
2.8
通讯作者：
Kandori, H
中科院分区：
文献类型：
--
作者：
Dozaki, D;Iwata, T;Kandori, H
In a plant blue-light receptor phototropin, the primary reaction is an adduct formation between the flavin chromophore and a cysteine S-H group. In the case of a LOV2 domain of Adiantum phytochrome3 (phy3-LOV2), we found that Arrhenius plots of the efficiency of the adduct formation provide two kinks at about 190 and 270 K, and a negative activation energy was obtained between the temperatures. They are close to the transition temperatures of protein structural changes observed by the low-temperature difference FTIR spectroscopy. We concluded that the observed temperature dependence of the reaction efficiency in a restricted protein environment originates from protein fluctuation that mainly reflects secondary structure. (c) 2005 Elsevier B.V. All rights reserved.
