Estimation of Hardy-Weinberg and pairwise disequilibrium in the apolipoprotein AI-CIII-AIV gene cluster.
Estimation of Hardy-Weinberg and pairwise disequilibrium in the apolipoprotein AI-CIII-AIV gene cluster.
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DOI：
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发表时间：
1991-08
影响因子：
9.8
通讯作者：
M. B. Haviland;A. Kessling;J. Davignon;C. Sing
中科院分区：
文献类型：
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作者：
M. B. Haviland;A. Kessling;J. Davignon;C. Sing
在对 apo AI-CIII-AIV 基因区域中的 6 个 RFLP 进行分类的不相关健康个体样本中，估计了 Hardy-Weinberg (HW) 平衡的偏离和成对不平衡。样本由男性和女性组成，从两个人群中选择健康，即纯法裔加拿大人 (FC) 和部分非法裔加拿大人 (NFC) 血统。使用了 Weir 和 Cockerham 提出的方法，其中包括对两个基因座上的三个和四个等位基因之间的非随机关联（不平衡）的估计以及两个基因座上的两个等位基因之间的传统关联的估计。偏离 HW 平衡的模式表明 FC 和 NFC 的遗传结构是不同的。无法根据同一基因区域中其他基因座的信息预测 RFLP 基因座偏离 HW 平衡的情况。从成对分析中也可以看出非随机关联。两对基因座具有显着的二基因不平衡，而另外两对具有显着的三基因不平衡。所有 RFLP 对至少具有一项由等位基因频率确定的最大值处的不平衡测量。关于成对不平衡的推论取决于所使用的统计方法。成对不平衡的大小与基因座之间的物理距离不相关。讨论了这些不平衡对 RFLP-表型关联研究的影响。
Departures from Hardy-Weinberg (HW) equilibria and pairwise disequilibria were estimated in a sample of unrelated healthy individuals typed for six RFLPs in the apo AI-CIII-AIV gene region. The sample was composed of males and females, selected for health, from two populations, those of exclusively French-Canadian (FC) and those of some non-French-Canadian (NFC) ancestry. An approach suggested by Weir and Cockerham, which includes estimates of nonrandom association (disequilibria) between three and four alleles at two loci as well as the traditional associations between two alleles, at two loci was used. The pattern of departures from HW equilibria suggested that the genetic structures of the FC and NFC are different. Departure from HW equilibrium at an RFLP locus could not be predicted from information about other loci in the same gene region. Nonrandom associations were also evident from the pairwise analyses. Two pairs of loci had significant diallelic disequilibria, while two other pairs had significant triallelic disequilibria. All of the RFLP pairs had at least one measure of disequilibrium at its maximum value determined by allele frequencies. Inferences about pairwise disequilibria depended on the statistical approach used. Sizes of the pairwise disequilibria were not correlated with the physical distance between loci. The impact of these disequilibria on RFLP-phenotype association studies is discussed.