Blue light emitting diodes irradiation causes cell death in colorectal cancer by inducing ROS production and DNA damage
Blue light emitting diodes irradiation causes cell death in colorectal cancer by inducing ROS production and DNA damage
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DOI：
10.1016/j.biocel.2018.08.006
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发表时间：
2018-10-01
影响因子：
4
通讯作者：
Yang, Lei
中科院分区：
文献类型：
--
作者：
Yan, Gege;Zhang, Lai;Yang, Lei
发光二极管（LED）照射已被证明是几种疾病的潜在治疗策略。然而，蓝光LED在结直肠癌（CRC）中的作用在很大程度上仍然未知，结直肠癌是世界各地发病率和死亡率的主要原因。在这项研究中，我们确定了蓝色LED照射的影响，在470 nm波长的最大光发射，在人CRC细胞系SW 620和HT 29。分别以0 J/cm（2）、72 J/cm（2）、144 J/cm（2）、216 J/cm（2）和288 J/cm（2）的蓝光照射细胞。我们发现用蓝色LED光照射诱导活细胞的显著减少和死细胞的增加。此外，与未照射对照组相比，蓝光LED照射组细胞增殖能力降低，细胞凋亡明显增加。蓝光LED照射24、48和72 h后，细胞迁移能力明显低于未照射组。蓝色LED处理的CRC细胞进一步显示出显着抑制CRC细胞中的EMT过程。最后，我们发现蓝光LED照射可导致ROS的产生和DNA损伤。这些结果表明，蓝光LED照射抑制CRC细胞的增殖，迁移和EMT过程，以及诱导细胞凋亡，这可能是由于增加ROS积累和诱导DNA损伤。
The light emitting diodes (LEDs) irradiation has been demonstrated to be potential therapeutic strategies for several diseases. However, the blue LED effects remain largely unknown in colorectal cancer (CRC), which is a major cause of morbidity and mortality throughout the world. In this study, we determined the effects of blue LED irradiation, the maximal light emission at 470 nm in wavelength, in human CRC cell lines SW620 and HT29. The cells were irradiated with blue LED light for 0 J/cm(2), 72 J/cm(2), 144 J/cm(2), 216 J/cm(2) and 288 J/cm(2) respectively. We found that irradiation with blue LED light induced a marked decrease of live cells and an increase of dead cells. Additionally, lower cell proliferation and a remarkably increase of cell apoptosis were observed in blue LED-irradiated cells as compared with non-irradiated control group. The cell migration was significantly inhibited by blue LED irradiation 24, 48 and 72 h later compared with non-treated group. Blue LED-treated CRC cells further displayed a remarkably inhibition of EMT process in CRC cells. Finally, we found the accumulation of ROS production and DNA damage were induced by blue LED irradiation. These results indicated that blue LED irradiation inhibits CRC cell proliferation, migration and EMT process as well as induces cell apoptosis, which may result from increased ROS accumulation and induction of DNA damage.