Extracting kinetic rate constants from surface plasmon resonance array systems
Extracting kinetic rate constants from surface plasmon resonance array systems
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DOI：
10.1016/j.ab.2007.08.017
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发表时间：
2008-02-01
影响因子：
2.9
通讯作者：
Myszka, David G.
中科院分区：
文献类型：
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作者：
Rich, Rebecca L.;Cannon, Michelle J.;Myszka, David G.
表面等离子共振成像系统，例如 Biacore 的 Flexchip，能够在单个流通池内同时监测数百个反应点。解释大型流通池中的结合动力学存在许多潜在的挑战，包括考虑质量传输效应和点对点样品消耗。我们采用计算机模拟和实验相结合来表征斑点阵列上的这些效应，并确定可以使用简单的两室模型在质量传输限制的条件下从阵列中准确提取固有速率常数。使用抗体系统，我们证明结合动力学的点与点之间的变异性是
Surface plasmon resonance imaging systems, such as Flexchip from Biacore, are capable of monitoring hundreds of reaction spots simultaneously within a single flow cell. Interpreting the binding kinetics in a large-format flow cell presents a number of potential challenges, including accounting for mass transport effects and spot-to-spot sample depletion. We employed a combination of computer simulations and experimentation to characterize these effects across the spotted array and established that a simple two-compartment model may be used to accurately extract intrinsic rate constants from the array under mass transport-limited conditions. Using antibody systems, we demonstrate that the spot-to-spot variability in the binding kinetics was