GENETIC AND ACTIVITY-DEPENDENT REGULATION OF ZIF268 EXPRESSION - ASSOCIATION WITH SPATIAL-LEARNING
GENETIC AND ACTIVITY-DEPENDENT REGULATION OF ZIF268 EXPRESSION - ASSOCIATION WITH SPATIAL-LEARNING
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DOI：
10.1002/hipo.450040505
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发表时间：
1994-10-01
期刊：
影响因子：
3.5
通讯作者：
WEHNER, JM
中科院分区：
文献类型：
--
作者：
FORDYCE, DE;BHAT, RV;WEHNER, JM
我们已经报道了C57 BL/6和DBA/2小鼠在空间学习表现和相关的海马蛋白激酶C（PKC）活性方面不同（Upchurch和Wehner，1989，Behav Neurosci 103：1251-1258; Wehner等人，1990，Brain Res 523：181-187），并且身体活动通过蛋白激酶C（PKC）的相关改变增强空间学习（福代斯和Wehner，1993 b，Brain Res 619：111-119）。为了评估体力活动是否会引起基因表达的改变，这可能是PKC和学习能力变化的基础，我们研究了体力活动对zif 268表达的影响，zif 268是一种与刺激诱导的神经元可塑性相关的转录调节因子。使3月龄的C57和DBA小鼠经受急性（一次）或慢性（8周）体力活动。然后在Morris水迷宫任务中测试小鼠6天，随后分析PKC活性和zif 268 mRNA表达。与C57小鼠相比，对照DBA小鼠具有较差的大脑特异性学习表现（Wehner等人，1990，Brain Res 523：181-187;福代斯和Wehner，1993 b，Brain Res 619：111-119; Paylor等人，1993，Psychobiology 27：11-26），显示zif 268 mRNA的基础水平较低（P
We have reported that C57BL/6 and DBA/2 mice differ in spatial learning performance and associated hippocampal protein kinase C (PKC) activity (Upchurch and Wehner, 1989, Behav Neurosci 103:1251-1258; Wehner et al., 1990, Brain Res 523:181-187) and that physical activity enhances spatial learning with related alterations in protein kinase C (PKC) (Fordyce and Wehner, 1993b, Brain Res 619:111-119). To assess whether physical activity induces alterations in gene expression that may underlie these changes in PKC and learning performance, we examined the effect of physical activity on expression of zif268, a transcription regulatory factor linked to stimulus-induced neuronal plasticity. C57 and DBA mice, 3 months of age, were subjected to acute (one bout) or chronic (8 weeks) physical activity. The mice were then tested on the Morris water maze task for 6 days with subsequent analysis of PKC activity and zif268 mRNA expression. Control DBA mice, which have poor hippocampal-specific learning performance compared to C57 mice (Wehner et al., 1990, Brain Res 523:181-187; Fordyce and Wehner, 1993b, Brain Res 619:111-119; Paylor et al., 1993, Psychobiology 27:11-26), displayed lower basal levels of zif268 mRNA (P