Light-up fluorophore-DNA aptamer pair for label-free turn-on aptamer sensors
Light-up fluorophore-DNA aptamer pair for label-free turn-on aptamer sensors
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DOI：
10.1039/c5cc08816j
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发表时间：
2016-01-01
影响因子：
4.9
通讯作者：
Hyuga, Masumi
中科院分区：
文献类型：
--
作者：
Kato, Teru;Shimada, Ippei;Hyuga, Masumi
我们开发了一对发光的荧光团-DNA适配子对，用于无标记适配子传感器，当与分析物结合时会发出荧光。与环境敏感的荧光团Dapoxyl结合的42聚体DNA适配子与分析物结合的DNA适配子融合后，荧光强度增加了700倍以上，成功地构建了适体传感器。
We developed a light-up fluorophore-DNA aptamer pair for label-free aptamer sensors that fluoresce upon binding to the analyte. A 42mer DNA aptamer binding to the environment-sensitive fluorophore, dapoxyl, which increased the fluorescence by more than 700-fold upon binding, was successfully used to construct aptamer sensors by fusion with analyte-binding DNA aptamers.