Comparative genomic hybridization is a powerful tool, complementary to cytogenetics, to identify chromosomal abnormalities in childhood acute lymphoblastic leukaemia
Comparative genomic hybridization is a powerful tool, complementary to cytogenetics, to identify chromosomal abnormalities in childhood acute lymphoblastic leukaemia
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DOI：
10.1046/j.1365-2141.1997.4243233.x
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发表时间：
1997-12-01
影响因子：
6.5
通讯作者：
AvetLoiseau, H
中科院分区：
文献类型：
--
作者：
PaszekVigier, M;Talmant, P;AvetLoiseau, H
细胞遗传学在儿童急性淋巴细胞白血病（ALL）中具有很强的预后价值，但由于染色体形态不佳，结果往往不完整。为了改善这种分析，我们测试了比较基因组杂交（CGH）检测染色体不平衡。采用CGH对72例儿童进行回顾性分析。只有53%的患者通过标准方法完全分带，用CGH，36例患者保留了正常的染色体轮廓，36例有不平衡的异常。对染色体核型不一致或不成功的病例，用着丝粒和独特序列探针进行荧光原位杂交（FISH），以验证CGH结果。CGH能够清楚地识别不平衡的染色体异常，即使在某些情况下，有一个正常的核型。鉴于超二倍体在儿童ALL中具有很强的预后价值，CGH似乎是一种强有力的技术，可作为常规细胞遗传学的补充。
Cytogenetics has a strong prognostic value in childhood acute lymphoblastic leukaemia (ALL), but results are often incomplete because of the poor chromosome morphology. To improve this analysis, we tested comparative genomic hybridization (CGH) for the detection of chromosomal imbalances. 72 children were retrospectively analysed using CGH. Only 53% of the patients had been fully banded by standard methods, With CGH, 36 patients retained a normal chromosomal profile and 36 had unbalanced abnormalities. No amplification was detected, Fluorescence in situ hybridization (FISH) with centromeric and unique sequence probes was used in those cases with discrepancies or unsuccessful karyotype to validate CGH results. CGH enabled clear identification of unbalanced chromosomal abnormalities, even in some cases which had a normal karyotype. In view of the strong prognostic value of hyperdiploidy in childhood ALL, CGH appears to be a powerful technique, complementary to conventional cytogenetics.