ESTROGEN-RECEPTOR EXPRESSION IN DEVELOPING EPIDIDYMIS, EFFERENT DUCTULES, AND OTHER MALE REPRODUCTIVE-ORGANS
ESTROGEN-RECEPTOR EXPRESSION IN DEVELOPING EPIDIDYMIS, EFFERENT DUCTULES, AND OTHER MALE REPRODUCTIVE-ORGANS
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DOI：
10.1210/endo-128-6-2874
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发表时间：
1991-06-01
期刊：
影响因子：
4.8
通讯作者：
CNHA, GR
中科院分区：
文献类型：
--
作者：
COOKE, PS;YOUNG, P;CNHA, GR
用H-3-雌二醇类固醇放射自显影技术测定了雄性BALB/c小鼠生殖器官中雌激素受体(ER)的分布。对16天(妊娠19-20天)至10日龄胎儿的传出小管、泌尿生殖窦和Wolffian导管及其衍生的附睾腺、输精管、精囊、凝固腺、前列腺和球部腺体进行了观察。所有胎儿生殖器官均强表达间充质ER。这些器官的基质细胞在以后的时间内仍保持ER+。然而，输精管、虫体、前列腺和附睾尾等器官中的平滑肌细胞在从间充质分化后仅呈弱ER+或ER-，而散布在平滑肌内的成纤维细胞仍呈强ER+。传出小管是发育中的男性生殖道中上皮性ER表达的第一个部位；该器官在妊娠第16天及随后表达上皮性ER。在妊娠第16天，Wolffian导管和尿路窦内未见上皮内质网。附精在分化后不久，即妊娠第19天开始表达上皮性ER。ER在附睾区的表达呈明显的梯度分布，其传出管和起始段的每个上皮细胞所含的银粒是更远端附睾区的3倍。精囊和凝固腺的上皮最初为ER-，但在出生后第6天及以后逐渐变为弱ER+。所有其他器官(输精管、前列腺和虫子)的上皮细胞在任何时候都不表达ER。
The distribution of estrogen receptors (ER) in developing reproductive organs of male BALB/c mice was determined by H-3-estradiol steroid autoradiography. Efferent ductules, urogenital sinus and Wolffian ducts, and their derivatives, the epididymis, ductus deferens, seminal vesicles, coagulating glands, prostate, and bulbouretheral glands (BUGs), were examined from 16 days fetal (gestation = 19-20 days) to 10 days postnatal. All fetal reproductive organs strongly expressed mesenchymal ER. Stromal cells of these organs remained ER+ at later times. However, smooth muscle cells in organs such as the ductus deferens, BUG, prostate, and caudal epididymis were only weakly ER+ or ER- after their differentiation from mesenchyme, although fibroblasts interspersed within the smooth muscle remained strongly ER+. Efferent ductules were the first site of epithelial ER expression in the developing male tract; this organ expressed epithelial ER on day 16 of gestation and subsequently. Wolffian ducts and urogential sinus did not contain epithelial ER on day 16 of gestation. Epididymis began expressing epithelial ER soon after its differentiation, on day 19 of gestation. A clear gradient of ER expression was noted in the regions of the developing epididymis, with the efferent ducts and the initial segment of the epididymis containing 3-fold more silver grains per epithelial cell than more distal regions of the epididymis. Epithelium of the seminal vesicle and coagulating gland was initially ER-, but became weakly ER+ at day 6 postnatal and later. The epithelium of all other organs (ductus deferens, prostate, and BUGs) never expressed ER at any time.