Metalloporphyrin speciation by liquid chromatography and inductively coupled plasma-mass spectrometry.
Metalloporphyrin speciation by liquid chromatography and inductively coupled plasma-mass spectrometry.
复制标题
通过液相色谱法和电感耦合等离子体质谱法进行金属卟啉形态分析。
DOI：
10.1093/chromsci/32.7.282
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发表时间：
1994
影响因子：
1.3
通讯作者：
Evans,EH
中科院分区：
文献类型：
--
作者：
Kumar,U;Dorsey,JG;Caruso,JA;Evans,EH
研究了电感耦合等离子体质谱法检测液相色谱法分离的金属卟啉（钴原卟啉、氯化血红素和锌原卟啉）。采用Hypersil SAS C1色谱柱，移动的流动相含68%甲醇，pH值为4.5。钴和锌原卟啉的检测限在纳克范围内，而氯化血红素的检测限在微克范围内。通过对一名铅中毒患者全血中锌原卟啉的测定，评价了该方法的准确性。
Inductively coupled plasma-mass spectrometry is investigated for the detection of metalloporphyrins (cobalt protoporphyrin, hemin, and zinc protoporphyrin) separated by liquid chromatography. A Hypersil SAS C1column with mobile phase containing 68% methanol at a pH of 4.5 is used. The detection limits obtained are in the nanogram range for cobalt and zinc protoporphyrins, whereas for hemin, detection limits are in the microgram range. The accuracy of the method is evaluated by determining zinc protoporphyrin from the whole blood of a lead-poisoned patient.