N-Sulfonyl-β-lactam hapten as an effective labeling reagent for aldolase mAb.
N-Sulfonyl-β-lactam hapten as an effective labeling reagent for aldolase mAb.
复制标题
N-磺酰基-β-内酰胺半抗原作为醛缩酶单克隆抗体的有效标记试剂。
DOI：
10.1016/j.bmcl.2015.03.011
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发表时间：
2015
影响因子：
2.7
通讯作者：
Barbas3rd,CarlosF
中科院分区：
文献类型：
--
作者：
Inokuma,Tsubasa;Fuller,RobertaP;Barbas3rd,CarlosF
利用化学程序化抗体（cpAbs）被认为是开发治疗系统的最有效方法之一。cpAb可以延长编程试剂的半衰期，通过抗体的Fc区激活免疫系统，并通过连接各种小的编程分子实现通用疫苗接种。本研究旨在开发一种新型的标记试剂用于制备cpAb，发现N-磺酰基-β-内酰胺（NSBL）是最佳的标记试剂。NSBL可由4-溴甲基苯磺酰氯经简单操作合成，并可标记用N-酰基-β-内酰胺（NABL）不能有效标记的醛缩酶单克隆抗体（mAb）84 G3。我们还证明了由mAb 84G3和带有生物素部分的NSBL组成的缀合物保持了与链霉亲和素的强结合活性。此外，NSBL的稳定性测定显示NSBL可耐受水性介质，24 h内无显著分解。
Utilization of chemically programmed antibodies (cpAbs) is regarded to be one of the most efficient methods for the development of therapeutic systems. cpAbs can extend the half-life of programming reagents, activate immune systems via the Fc region of antibodies and achieve universal vaccination by attaching varieties of small, programmed molecules. In the current study, we aimed to develop a novel labeling reagent for the preparation of cpAbs and found thatN-sulfonyl-β-lactams (NSBLs) were optimal. NSBL can be synthesized from readily available 4-(bromomethyl)benzenesulfonyl chloride via few simple manipulations and can label the aldolase monoclonal antibody (mAb) 84G3, which could not be labeled effectively by the conventional labeling reagent,N-acyl-β-lactam (NABL). We also demonstrated that the conjugate, which consists of mAb 84G3 and an NSBL bearing a biotin moiety, maintained strong binding activity to streptavidin. In addition, the stability assay of NSBL revealed that NSBLs can tolerate aqueous media without significant decomposition over 24 h.