Quantitative reverse transcription-PCR assay of the RNA component of human telomerase using the TaqMan fluorogenic detection system
Quantitative reverse transcription-PCR assay of the RNA component of human telomerase using the TaqMan fluorogenic detection system
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发表时间：
1998
影响因子：
2.7
通讯作者：
T. Yajima;A. Yagihashi;H. Kameshima;D. Kobayashi;D. Furuya;K. Hirata;N. Watanabe
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文献类型：
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作者：
T. Yajima;A. Yagihashi;H. Kameshima;D. Kobayashi;D. Furuya;K. Hirata;N. Watanabe
我们建立了一个有效的定量逆转录（RT）-PCR检测的RNA成分的人端粒酶（hTR），使用TaqMan荧光检测系统。使用该测定，我们定量了两种人胰腺癌细胞系ASPC-1和MIAPaCa-2中的hTR表达。我们的结果表明，MIAPaCa-2细胞中hTR的表达是ASPC-1细胞中hTR表达的1.99倍。该TaqMan RT-PCR测定似乎可用于确定临床标本中hTR的量。
We established the validity of a quantitative reverse transcription (RT)-PCR assay for the RNA component of human telomerase (hTR), using the TaqMan fluorogenic detection system. Using this assay, we quantified hTR expression in two human pancreatic cancer cell lines, ASPC-1 and MIAPaCa-2. Our results indicated that hTR expression in MIAPaCa-2 was 1.99-fold higher than that in ASPC-1 cells. This TaqMan RT-PCR assay appears to be useful in determining the amount of hTR in clinical specimens.