Genetic diversity of Plasmodium falciparum and its relationship to parasite density in an area with different malaria endemicities in West Uganda
Genetic diversity of Plasmodium falciparum and its relationship to parasite density in an area with different malaria endemicities in West Uganda
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DOI：
10.1046/j.1365-3156.2001.00761.x
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发表时间：
2001-08-01
影响因子：
3.3
通讯作者：
von Sonnenburg, F
中科院分区：
文献类型：
--
作者：
Peyerl-Hoffmann, G;Jelinek, T;von Sonnenburg, F
通过聚合酶链式反应扩增裂殖子表面蛋白1和2(MSP1和MSP2)的选定片段，可以有效地对恶性疟原虫野外种群进行基因分型。调查了乌干达西部卡巴罗勒地区不同传播水平地区恶性疟原虫种群的遗传多样性。对225例恶性疟原虫阳性标本进行了多态区扩增分析，并按等位基因家系的分布情况进行分类。每个座位均检测到大量等位基因：MSPI第2区22个，MSP2 24个。在2~8个克隆范围内，有175个(78%)MSP1等位基因和143个(%)MSP2表现出多重感染。恶性疟原虫全流行区与中流行区在种群结构和多克隆感染数量方面差异不明显。然而，在单克隆性感染和多克隆性感染中，寄生虫密度、选定的MSP2基因座和寄生虫密度之间存在显著的相关性。感染的多样性与年龄有关。
Field populations of Plasmodium falciparum can be effectively genotyped by PCR-amplification of selected fragments of the Merozoite Surface Proteins 1 and 2 (MSP1 and MSP2). Genetic diversity of P. falciparum populations in areas with different transmission levels (holo- vs. mesoendemic) was investigated in Kabarole District, West Uganda. 225 samples positive for P. falciparum were analysed by amplification of polymorphic regions and classified according to prevalence of allelic families. A large number of alleles was detected for each locus: 22 for MSPI block 2 and 24 for MSP2 and, 175 (78%) of MSP1 alleles and 143 (64%) of MSP2 showed multiple infections within a range of 2-8 clones. Significant differences between holoendemic and mesoendemic areas in regards of population structure and number of multiclonal infections of P. falciparum were not apparent. However, a significant correlation between parasite density, selected MSP2 loci and differences between parasite density in monoclonal vs. multiclonal infections occurred. Multiplicity of infection was age-dependent.