Low-temperature plasma promotes growth of Haematococcus pluvialis and accumulation of astaxanthin by regulating histone H3 lysine 4 tri-methylation
Low-temperature plasma promotes growth of Haematococcus pluvialis and accumulation of astaxanthin by regulating histone H3 lysine 4 tri-methylation
复制标题
低温血浆通过调节组蛋白H3赖氨酸4三甲基化促进雨生红球藻生长和虾青素积累
DOI：
10.1016/j.biortech.2021.126095
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发表时间：
2021-10-09
影响因子：
11.4
通讯作者：
Huang, Qing
中科院分区：
文献类型：
--
作者：
Li, Lamei;Chen, Zhu;Huang, Qing
虾青素具有很强的抗氧化能力，因此研究人员努力提高雨红球菌（H. pluvialis）虾青素的产量。以往的研究表明低温等离子体（LTP）可以提高雨螺虾青素的产量，但其机制尚不明确。因此，我们从表观遗传学的角度探讨了LTP促进藻类生长虾青素产量的机制。通过对激素和转录基因的测定，发现LTP处理后雨水杨体内的独角麦内酯和脱落酸水平显著升高，虾青素合成基因表达增强。特别是其中一个关键基因，即CRTISO，被特异性上调。进一步通过免疫荧光和ChIP-PCR方法证实，CRTISO启动子区组蛋白H3赖氨酸4三甲基化（H3K4me3）升高。因此，本研究表明LTP可以通过刺激植物激素和调节H3K4me3来调节CRTISO，促进藻类生长和虾青素积累。
Astaxanthin exhibits strong antioxidant ability, so researchers endeavor to improve astaxanthin production in Haematococcus pluvialis (H. pluvialis). Previous work revealed that low-temperature plasma (LTP) could improve the astaxanthin yield in H. pluvialis, but the mechanism is still elusive. In this work, we therefore explored the mechanism of LTP promoting algal growth astaxanthin yield, especially from the perspective of epigenetics. Through measurements of hormones and transcription genes, it was found that the levels of strigolactone and abscisic acid in H. pluvialis increased significantly after LTP treatment, accompanied by enhanced expression of astaxanthin synthesis genes. Particularly, one of the key genes, namely CRTISO, was specifically up-regulated. Further experiments via immunofluorescence and ChIP-PCR methods confirmed that histone H3 lysine 4 trimethylation (H3K4me3) in the promoter region of CRTISO was increased. Therefore, this study demonstrates that LTP can regulate CRTISO and promote the algal growth and astaxanthin accumulation by stimulating phytohormones and regulating H3K4me3.