The kinetics of thymine dimer excision in ultraviolet-irradiated human cells.
The kinetics of thymine dimer excision in ultraviolet-irradiated human cells.
复制标题
紫外线照射的人体细胞中胸腺嘧啶二聚体切除的动力学。
DOI：
10.1016/s0006-3495(78)85487-3
复制
发表时间：
1978
影响因子：
3.4
通讯作者：
E. Friedberg
中科院分区：
文献类型：
--
作者：
Ursula K. Ehmann;K. Cook;E. Friedberg
我们研究了几种紫外线（UV）照射培养的人细胞系的酸不溶性部分的胸腺嘧啶二聚体的损失的动力学。我们的研究结果表明，10和40 J/m2之间的紫外线通量产生平均21-85 × 10（5）胸腺嘧啶二聚体每个细胞和最终的最大损失12-20 × 10（5）胸腺嘧啶二聚体每个细胞。二聚体的半最大损失的时间范围从12-22小时后，紫外线照射。与此相反，通过放射自显影法测量的DNA半最大修复合成时间为4.5 h。该图与报道的半最大修复合成时间一致，根据我们的分析，其范围为0.5至3.6小时。胸腺嘧啶二聚体从DNA和修复合成的损失的动力学的差异进行了讨论，在胸腺嘧啶二聚体切除在体内的可能的分子机制，并在可能的实验文物。
We have investigated the kinetics of the loss of thymine dimers from the acid-insoluble fraction of several ultraviolet (UV)-irradiated cultured human cell lines. Our results show that UV fluences between 10 and 40 J/m2 produce an average of 21–85 x 10(5) thymine dimers per cell and an eventual maximal loss per cell of 12–20 x 10(5) thymine dimers. The time for half-maximal loss of dimers ranged from 12–22 h after UV irradiation. In contrast, the time for half-maximal repair synthesis of DNA measured by autoradiography was 4.5 h. This figure agrees well with reported half-maximal repair synthesis times, which range from 0.5 to 3.6 h based on our analysis. The discrepancy in the kinetics of the loss of thymine dimers from DNA and repair synthesis is discussed in terms of possible molecular mechanisms of thymine dimer excision in vivo and in terms of possible experimental artifacts.