The critical requirement for linolenic acid is pollen development, not photosynthesis, in an arabidopsis mutant
The critical requirement for linolenic acid is pollen development, not photosynthesis, in an arabidopsis mutant
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DOI：
10.1105/tpc.8.3.403
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发表时间：
1996-03-01
期刊：
影响因子：
11.6
通讯作者：
Browse, J
中科院分区：
文献类型：
--
作者：
McConn, M;Browse, J
在所有高等植物的叶绿体膜中发现了非常高比例的三烯脂肪酸，这表明这些脂质结构可能是光合作用所必需的。我们在这里报告的生产拟南芥三重突变体，含有可忽略不计的三烯脂肪酸。在22摄氏度的光合作用几乎没有受到影响，和营养生长的突变体是相同的野生型，表明在膜结构和功能的三烯酰基基团的任何要求是相对微妙的。虽然营养生长和发育不受影响，但三重突变体是雄性不育的，在正常条件下不产生种子。野生型和三重突变体花的花粉发育的比较建立了突变体中的花粉粒发育到三细胞阶段。外源应用α-亚麻酸或茉莉酸恢复生育力。两者合计，结果表明，三烯酸在植物的生命周期中的关键作用是作为oxlipin，一种信号化合物，调节最终成熟过程和花粉的释放的前体。
The very high proportions of trienoic fatty acids found in chloroplast membranes of all higher plants suggest that these lipid structures might be essential for photosynthesis. We report here on the production of Arabidopsis triple mutants that contain negligible levels of trienoic fatty acids. Photosynthesis at 22 degrees C was barely affected, and vegetative growth of the mutants was identical with that of the wild type, demonstrating that any requirement for trienoic acyl groups in membrane structure and function is relatively subtle. Although vegetative growth and development were unaffected, the triple mutants are male sterile and produce no seed under normal conditions. Comparisons of pollen development in wild-type and triple mutant flowers established that pollen grains in the mutant developed to the tricellular stage. Exogenous applications of alpha-linolenate or jasmonate restored fertility. Taken together, the results demonstrate that the critical role of trienoic acids in the life cycle of plants is as the precursor of oxylipin, a signaling compound that regulates final maturation processes and the release of pollen.